体外组装的甲病毒核心样颗粒维持着与成熟病毒中核衣壳核心相似的结构。
In vitro-assembled alphavirus core-like particles maintain a structure similar to that of nucleocapsid cores in mature virus.
作者信息
Mukhopadhyay Suchetana, Chipman Paul R, Hong Eunmee M, Kuhn Richard J, Rossmann Michael G
机构信息
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA.
出版信息
J Virol. 2002 Nov;76(21):11128-32. doi: 10.1128/jvi.76.21.11128-11132.2002.
Abstract
In vitro-assembled core-like particles produced from alphavirus capsid protein and nucleic acid were studied by cryoelectron microscopy. These particles were found to have a diameter of 420 A with 240 copies of the capsid protein arranged in a T=4 icosahedral surface lattice, similar to the nucleocapsid core in mature virions. However, when the particles were subjected to gentle purification procedures, they were damaged, preventing generation of reliable structural information. Similarly, purified nucleocapsid cores isolated from virus-infected cells or from mature virus particles were also of poor quality. This suggested that in the absence of membrane and glycoproteins, nucleocapsid core particles are fragile, lacking accurate icosahedral symmetry.
摘要
利用冷冻电子显微镜对由甲病毒衣壳蛋白和核酸体外组装而成的核心样颗粒进行了研究。发现这些颗粒直径为420埃，有240个衣壳蛋白拷贝排列成T = 4二十面体表面晶格，类似于成熟病毒粒子中的核衣壳核心。然而，当对这些颗粒进行温和的纯化程序时，它们受到了损伤，从而无法生成可靠的结构信息。同样，从病毒感染细胞或成熟病毒粒子中分离出的纯化核衣壳核心质量也很差。这表明在没有膜和糖蛋白的情况下，核衣壳核心颗粒很脆弱，缺乏精确的二十面体对称性。
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本文引用的文献
1
Placement of the structural proteins in Sindbis virus.辛德毕斯病毒中结构蛋白的定位
J Virol. 2002 Nov;76(22):11645-58. doi: 10.1128/jvi.76.22.11645-11658.2002.
2
Aura virus structure suggests that the T=4 organization is a fundamental property of viral structural proteins.奥罗病毒的结构表明，T=4结构是病毒结构蛋白的基本特性。
J Virol. 2002 Jul;76(14):7239-46. doi: 10.1128/jvi.76.14.7239-7246.2002.
3
Genetic and biochemical studies on the assembly of an enveloped virus.关于包膜病毒组装的遗传学和生物化学研究。
Genet Eng (N Y). 2001;23:83-112. doi: 10.1007/0-306-47572-3_6.
4
Venezuelan equine encephalomyelitis virus structure and its divergence from old world alphaviruses.委内瑞拉马脑炎病毒的结构及其与旧大陆甲病毒的差异。
J Virol. 2001 Oct;75(19):9532-7. doi: 10.1128/JVI.75.19.9532-9537.2001.
5
The Fusion glycoprotein shell of Semliki Forest virus: an icosahedral assembly primed for fusogenic activation at endosomal pH.塞姆利基森林病毒的融合糖蛋白外壳：一种在内体pH值下准备进行融合激活的二十面体装配体。
Cell. 2001 Apr 6;105(1):137-48. doi: 10.1016/s0092-8674(01)00303-8.
6
Locations of carbohydrate sites on alphavirus glycoproteins show that E1 forms an icosahedral scaffold.甲病毒糖蛋白上碳水化合物位点的位置表明，E1形成一个二十面体支架。
Cell. 2001 Apr 6;105(1):127-136. doi: 10.1016/s0092-8674(01)00302-6.
7
Alphavirus nucleocapsid protein contains a putative coiled coil alpha-helix important for core assembly.甲病毒核衣壳蛋白包含一个对核心组装很重要的假定卷曲螺旋α螺旋。
J Virol. 2001 Jan;75(1):1-10. doi: 10.1128/JVI.75.1.1-10.2001.
8
Membrane proteins organize a symmetrical virus.膜蛋白构成了对称的病毒。
EMBO J. 2000 Oct 2;19(19):5081-91. doi: 10.1093/emboj/19.19.5081.
9
Cryo-electron microscopy reveals the functional organization of an enveloped virus, Semliki Forest virus.冷冻电子显微镜揭示了包膜病毒——辛德毕斯病毒的功能组织。
Mol Cell. 2000 Feb;5(2):255-66. doi: 10.1016/s1097-2765(00)80421-9.
10
Nucleic acid-dependent cross-linking of the nucleocapsid protein of Sindbis virus.辛德毕斯病毒核衣壳蛋白的核酸依赖性交联
J Virol. 2000 May;74(9):4302-9. doi: 10.1128/jvi.74.9.4302-4309.2000.
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