Deng Chunhui, Deng Yonghui, Wang Bin, Yang Xiuhan
Department of Chemistry, Fudan University, Shanghai 200433, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Nov 25;780(2):407-13. doi: 10.1016/s1570-0232(02)00632-3.
In this paper we developed a simple, rapid and sensitive method for the quantitative analysis of phenylalanine (Phe) and tyrosine (Tyr) in dried blood spots of newborns by gas chromatography-mass spectrometry (GC-MS). Phe and Tyr in blood samples were reacted with N-methyl-N-(tert.-butyldimethylsilyl)trifluoroacetamide at 120 degrees C for 30 min and their corresponding single derivatives were obtained. Phe and Tyr were determined by measurement of their derivatives by GC-MS in the selected ion monitoring mode. Contents of Phe and Tyr in blood spots were calculated by external standard method. The ratio of Phe to Tyr was used for neonatal screening for phenylketonuria. The present method only took a few minutes to perform and required minimal sample preparation. In addition it provided low detection limits of 1.2 micromol l(-1) for Phe and 1.6 micromol l(-1)for Tyr.
在本文中，我们开发了一种简单、快速且灵敏的方法，用于通过气相色谱 - 质谱联用（GC - MS）对新生儿干血斑中的苯丙氨酸（Phe）和酪氨酸（Tyr）进行定量分析。血样中的Phe和Tyr在120℃下与N - 甲基 - N - （叔丁基二甲基甲硅烷基）三氟乙酰胺反应30分钟，得到它们相应的单一衍生物。通过在选择离子监测模式下用GC - MS测量其衍生物来测定Phe和Tyr。血斑中Phe和Tyr的含量通过外标法计算。Phe与Tyr的比值用于苯丙酮尿症的新生儿筛查。本方法只需几分钟即可完成，且所需样品制备极少。此外，它对Phe的检测限低至1.2微摩尔/升，对Tyr的检测限为1.6微摩尔/升。
