内含子状态和3'端形成控制mRNA的共转录输出。
Intron status and 3'-end formation control cotranscriptional export of mRNA.
作者信息
Lei Elissa P, Silver Pamela A
机构信息
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School and Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.
出版信息
Genes Dev. 2002 Nov 1;16(21):2761-6. doi: 10.1101/gad.1032902.
Abstract
Messenger RNA export factors are recruited to genes in a transcription-dependent manner. To ascertain the mechanism of this process, we show that RNA polymerase II transcription is sufficient to recruit the Saccharomyces cerevisiae hnRNP protein Npl3 to a gene independent of RNA sequence. In contrast, the cotranscriptional recruitment of the RNA-binding protein Yra1 is dependent on pre-mRNA processing. Yra1 associates with introns of intron-containing genes in a splicing-dependent manner. Conversely, Yra1 recruitment to genes without introns is not dependent on splicing. Finally, 3'-end formation is required for Yra1 recruitment to genes regardless of intron status.
摘要
信使核糖核酸（mRNA）输出因子以转录依赖的方式被招募到基因上。为了确定这一过程的机制，我们发现RNA聚合酶II转录足以将酿酒酵母的异质性核糖核蛋白（hnRNP）Npl3蛋白招募到一个与RNA序列无关的基因上。相比之下，RNA结合蛋白Yra1的共转录招募依赖于前体mRNA加工。Yra1以剪接依赖的方式与含内含子基因的内含子结合。相反，Yra1被招募到无内含子基因上则不依赖于剪接。最后，无论内含子状态如何，Yra1被招募到基因上都需要3'端形成。
相似文献
1
Intron status and 3'-end formation control cotranscriptional export of mRNA.内含子状态和3'端形成控制mRNA的共转录输出。
Genes Dev. 2002 Nov 1;16(21):2761-6. doi: 10.1101/gad.1032902.
2
Messenger RNAs are recruited for nuclear export during transcription.信使核糖核酸在转录过程中被招募用于核输出。
Genes Dev. 2001 Jul 15;15(14):1771-82. doi: 10.1101/gad.892401.
3
Differential recruitment of the splicing machinery during transcription predicts genome-wide patterns of mRNA splicing.转录过程中剪接机制的差异募集预测全基因组范围内的mRNA剪接模式。
Mol Cell. 2006 Dec 28;24(6):903-15. doi: 10.1016/j.molcel.2006.12.006.
4
TREX is a conserved complex coupling transcription with messenger RNA export.转录-输出衔接复合体（TREX）是一种保守的复合体，它将转录与信使核糖核酸输出相偶联。
Nature. 2002 May 16;417(6886):304-8. doi: 10.1038/nature746. Epub 2002 Apr 28.
5
Tho1, a novel hnRNP, and Sub2 provide alternative pathways for mRNP biogenesis in yeast THO mutants.Tho1是一种新型异质性核糖核蛋白，Sub2为酵母THO突变体中的信使核糖核蛋白生物合成提供了替代途径。
Mol Cell Biol. 2006 Jun;26(12):4387-98. doi: 10.1128/MCB.00234-06.
6
A subset of Mer1p-dependent introns requires Bud13p for splicing activation and nuclear retention.一部分依赖Mer1p的内含子需要Bud13p来激活剪接和核保留。
RNA. 2006 Jul;12(7):1361-72. doi: 10.1261/rna.2276806. Epub 2006 May 31.
7
Genome-wide analysis of RNA-protein interactions illustrates specificity of the mRNA export machinery.全基因组范围内的RNA-蛋白质相互作用分析揭示了mRNA输出机制的特异性。
Nat Genet. 2003 Feb;33(2):155-61. doi: 10.1038/ng1080. Epub 2003 Jan 13.
8
Expression of the essential mRNA export factor Yra1p is autoregulated by a splicing-dependent mechanism.必需的mRNA输出因子Yra1p的表达通过一种剪接依赖性机制进行自我调节。
RNA. 2002 Aug;8(8):969-80. doi: 10.1017/s1355838202020046.
9
Cotranscriptional recruitment of the serine-arginine-rich (SR)-like proteins Gbp2 and Hrb1 to nascent mRNA via the TREX complex.富含丝氨酸-精氨酸的（SR）样蛋白Gbp2和Hrb1通过TREX复合物共转录募集到新生mRNA上。
Proc Natl Acad Sci U S A. 2004 Feb 17;101(7):1858-62. doi: 10.1073/pnas.0308663100. Epub 2004 Feb 9.
10
The yeast hnRNP-Like proteins Yra1p and Yra2p participate in mRNA export through interaction with Mex67p.酵母类异质性核糖核蛋白Yra1p和Yra2p通过与Mex67p相互作用参与mRNA输出。
Mol Cell Biol. 2001 Jul;21(13):4219-32. doi: 10.1128/MCB.21.13.4219-4232.2001.
引用本文的文献
1
FXR1 can bind with the CFIm25/CFIm68 complex and promote the progression of urothelial carcinoma of the bladder by stabilizing TRAF1 mRNA.FXR1 可以与 CFIm25/CFIm68 复合物结合，并通过稳定 TRAF1 mRNA 促进膀胱尿路上皮癌的进展。
Cell Death Dis. 2022 Feb 22;13(2):170. doi: 10.1038/s41419-022-04614-1.
2
Distinct Functions of the Cap-Binding Complex in Stimulation of Nuclear mRNA Export.帽结合复合物在刺激核 mRNA 输出中的独特功能。
Mol Cell Biol. 2019 Apr 2;39(8). doi: 10.1128/MCB.00540-18. Print 2019 Apr 15.
3
Quality control of mRNAs at the entry of the nuclear pore: Cooperation in a complex molecular system.核孔入口处 mRNA 的质量控制：复杂分子系统中的合作。
Nucleus. 2018 Jan 1;9(1):202-211. doi: 10.1080/19491034.2018.1439304.
4
Regulation of RNA-binding proteins affinity to export receptors enables the nuclear basket proteins to distinguish and retain aberrant mRNAs.RNA 结合蛋白与输出受体亲和力的调控使核篮蛋白能够区分并保留异常 mRNA。
Sci Rep. 2016 Nov 2;6:35380. doi: 10.1038/srep35380.
5
Interactions between KSHV ORF57 and the novel human TREX proteins, CHTOP and CIP29.卡波西肉瘤相关疱疹病毒ORF57与新型人类转录/输出复合物（TREX）蛋白CHTOP和CIP29之间的相互作用
J Gen Virol. 2016 Aug;97(8):1904-1910. doi: 10.1099/jgv.0.000503. Epub 2016 May 16.
6
Upregulation of RNA Processing Factors in Poorly Differentiated Lung Cancer Cells.RNA 加工因子在低分化肺癌细胞中的上调。
Transl Oncol. 2016 Apr;9(2):89-98. doi: 10.1016/j.tranon.2016.01.006. Epub 2016 Mar 4.
7
Nervous translation, do you get the message? A review of mRNPs, mRNA-protein interactions and translational control within cells of the nervous system.神经翻译，你明白其中的信息吗？对神经系统细胞内的信使核糖核蛋白颗粒、信使核糖核酸-蛋白质相互作用及翻译控制的综述。
Cell Mol Life Sci. 2014 Oct;71(20):3917-37. doi: 10.1007/s00018-014-1660-x. Epub 2014 Jun 22.
8
A novel RNA motif mediates the strict nuclear localization of a long noncoding RNA.一种新型RNA基序介导长链非编码RNA的严格核定位。
Mol Cell Biol. 2014 Jun;34(12):2318-29. doi: 10.1128/MCB.01673-13. Epub 2014 Apr 14.
9
Life of psi: how full-length HIV-1 RNAs become packaged genomes in the viral particles.psi 生活：全长 HIV-1 RNA 如何成为病毒颗粒中的包装基因组。
Virology. 2014 Apr;454-455:362-70. doi: 10.1016/j.virol.2014.01.019. Epub 2014 Feb 14.
10
RNA polymerase II C-terminal domain: Tethering transcription to transcript and template.RNA聚合酶II羧基末端结构域：将转录与转录本和模板相连
Chem Rev. 2013 Nov 13;113(11):8423-55. doi: 10.1021/cr400158h. Epub 2013 Sep 16.
本文引用的文献
1
The mRNA assembly line: transcription and processing machines in the same factory.信使核糖核酸装配线：同一工厂内的转录与加工机器
Curr Opin Cell Biol. 2002 Jun;14(3):336-42. doi: 10.1016/s0955-0674(02)00333-2.
2
Functional interaction of yeast pre-mRNA 3' end processing factors with RNA polymerase II.酵母前体mRNA 3' 末端加工因子与RNA聚合酶II的功能相互作用。
Mol Cell. 2002 May;9(5):1101-11. doi: 10.1016/s1097-2765(02)00518-x.
3
TREX is a conserved complex coupling transcription with messenger RNA export.转录-输出衔接复合体（TREX）是一种保守的复合体，它将转录与信使核糖核酸输出相偶联。
Nature. 2002 May 16;417(6886):304-8. doi: 10.1038/nature746. Epub 2002 Apr 28.
4
Integrating mRNA processing with transcription.将信使核糖核酸加工与转录整合
Cell. 2002 Feb 22;108(4):501-12. doi: 10.1016/s0092-8674(02)00617-7.
5
Protein and RNA export from the nucleus.蛋白质和RNA从细胞核输出。
Dev Cell. 2002 Mar;2(3):261-72. doi: 10.1016/s1534-5807(02)00134-x.
6
Stimulatory effect of splicing factors on transcriptional elongation.剪接因子对转录延伸的刺激作用。
Nature. 2001;414(6866):929-33. doi: 10.1038/414929a.
7
Splicing factor Sub2p is required for nuclear mRNA export through its interaction with Yra1p.剪接因子Sub2p通过与Yra1p相互作用，对核mRNA输出是必需的。
Nature. 2001 Oct 11;413(6856):648-52. doi: 10.1038/35098113.
8
Pre-mRNA splicing and mRNA export linked by direct interactions between UAP56 and Aly.UAP56与Aly之间的直接相互作用将前体mRNA剪接与mRNA输出联系起来。
Nature. 2001 Oct 11;413(6856):644-7. doi: 10.1038/35098106.
9
Quality control of mRNA 3'-end processing is linked to the nuclear exosome.mRNA 3'端加工的质量控制与核外切体相关。
Nature. 2001 Oct 4;413(6855):538-42. doi: 10.1038/35097110.
10
The exon-exon junction complex provides a binding platform for factors involved in mRNA export and nonsense-mediated mRNA decay.外显子-外显子连接复合体为参与mRNA输出和无义介导的mRNA降解的因子提供了一个结合平台。
EMBO J. 2001 Sep 3;20(17):4987-97. doi: 10.1093/emboj/20.17.4987.
Zotero 插件