Kalauni Surya K, Choudhary Muhammad Iqbal, Khalid Asaad, Manandhar Mangala Devi, Shaheen Farzana, Gewali Mohan Bikram
Central Department of Chemistry, Tribhuvan University, Nepal.
Chem Pharm Bull (Tokyo). 2002 Nov;50(11):1423-6. doi: 10.1248/cpb.50.1423.
From the leaves of Sarcococca coriacea two new steroidal alkaloids, epoxynepapakistamine-A [(20S)-20-(N-methylamino)-3beta-(tigloylamino)-5alpha-pregna-16alpha,17alpha-epoxy-2beta,4beta-di-O-acetate] (1), and epoxysarcovagenine-D [(20S)-20-(N-methylamino)-3beta-(tigloylamino)-5alpha-pregna-2-en-16alpha,17alpha-epoxy-4-one] (2), and two known compounds funtumafrine C [(20S)-20-(N,N-dimethylamino)-5alpha-pregna-3-one] (3) and N-methylfuntumine (4) were isolated. Their structures were elucidated on the basis of their spectral properties. The compounds 1, 3 and 4 were found to have cholinesterase inhibitory activity when tested for the inhibition of electric eel acetylcholinesterase and horse serum butyrylcholinesterase. They inhibited both enzymes in a concentration dependent fashion.
从厚叶清香桂的叶子中分离出两种新的甾体生物碱，环氧新帕基胺 -A [（20S）-20-（N-甲基氨基）-3β-（惕各酰氨基）-5α-娠烷 -16α，17α-环氧 -2β，4β-二 -O-乙酸酯]（1）和环氧肉珊瑚宁-D [（20S）-20-（N-甲基氨基）-3β-（惕各酰氨基）-5α-娠烷 -2-烯 -16α，17α-环氧 -4-酮]（2），以及两种已知化合物丰土马弗林C [（20S）-20-（N，N-二甲基氨基）-5α-娠烷 -3-酮]（3）和N-甲基丰土明（4）。根据它们的光谱性质阐明了它们的结构。当测试对电鳗乙酰胆碱酯酶和马血清丁酰胆碱酯酶的抑制作用时，发现化合物1、3和4具有胆碱酯酶抑制活性。它们以浓度依赖性方式抑制这两种酶。
