与培养的哺乳动物细胞核相关的粘多糖
Mucopolysaccharides associated with nuclei of cultured mammalian cells.
作者信息
Bhavanandan V P, Davidson E A
出版信息
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2032-6. doi: 10.1073/pnas.72.6.2032.
Abstract
Mucopolysaccharides have been isolated, fractionated, and characterized from the nuclei of cultured B16 mouse melanoma cells grown in the presence of (3-H)-glucosamine and (35-S)sulfate. Digestion of the nuclei with DNase followed by Pronase gave a mixture of complex carbohydrates from which the mucopolysaccharides were isolated by precipitation with cetylpyridinium chloride. After fractionation by differential salt extraction and chromatography on controlled pore glass bead columns, the components were identified by chemical and enzymatic methods. The major polysaccharide components were a family of high-molecular-weight chondroitin sulfates with different degrees of sulfation; a minor component has been characterized as heparan sulfate.
摘要
在含有（3 - H）-葡萄糖胺和（35 - S）-硫酸盐的条件下培养的B16小鼠黑色素瘤细胞核中，已分离、分级并鉴定了粘多糖。先用脱氧核糖核酸酶消化细胞核，再用链霉蛋白酶处理，得到一种复合碳水化合物混合物，通过用十六烷基氯化吡啶沉淀从中分离出粘多糖。通过差示盐提取和在可控孔径玻璃珠柱上进行色谱分离后，用化学和酶学方法鉴定了各组分。主要的多糖组分是一族具有不同硫酸化程度的高分子量硫酸软骨素；一种次要组分已被鉴定为硫酸乙酰肝素。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Tissue fractionation studies. 5. The association of acid phosphatase with a special class of cytoplasmic granules in rat liver.组织分级分离研究。5. 大鼠肝脏中酸性磷酸酶与一类特殊细胞质颗粒的关联。
Biochem J. 1955 Mar;59(3):438-45. doi: 10.1042/bj0590438.
3
CHANGES IN THE MITOTIC INDEX OF THE LANDSCHUETZ ASCITES TUMOUR AFTER TREATMENT WITH TUMOUR-INHIBITORY OR NON-INHIBITORY SAMPLES OF GUMTRAGACANTH OR WITH GUM KARAYA.用刺梧桐树胶或卡拉牙胶的抑瘤或非抑瘤样品处理后兰氏腹水瘤有丝分裂指数的变化
Br J Cancer. 1964 Sep;13(3):528-36. doi: 10.1038/bjc.1964.60.
4
Determination of 2-deoxy-2-sulfoaminohexose content of mucopolysaccharides.黏多糖中2-脱氧-2-磺氨基己糖含量的测定
Arch Biochem Biophys. 1962 Dec;99:396-400. doi: 10.1016/0003-9861(62)90285-0.
5
Biochemistry of dystrophic muscle. Mitochondrial succinate-tetrazolium reductase and adenosine triphosphatase.营养不良性肌肉的生物化学。线粒体琥珀酸 - 四氮唑还原酶和三磷酸腺苷酶。
Biochem J. 1961 Sep;80(3):649-54. doi: 10.1042/bj0800649.
6
Mucopolysaccharides produced in tissue culture.组织培养中产生的粘多糖。
J Biophys Biochem Cytol. 1957 May 25;3(3):391-6. doi: 10.1083/jcb.3.3.391.
7
Studies on the terminal electron transport system. I. Succinic dehydrogenase.末端电子传递系统的研究。I. 琥珀酸脱氢酶。
J Biol Chem. 1955 Dec;217(2):551-67.
8
Nuclei from rat liver: isolation method that combines purity with high yield.大鼠肝脏细胞核：一种将纯度与高产率相结合的分离方法。
Science. 1966 Dec 30;154(3757):1662-5. doi: 10.1126/science.154.3757.1662.
9
Heparin as a possible initiator of genomic RNA synthesis in early development of sea urchin embryos.肝素作为海胆胚胎早期发育中基因组RNA合成的可能启动因子。
Exp Cell Res. 1971 Feb;64(2):403-11. doi: 10.1016/0014-4827(71)90094-2.
10
Recent developments in the measurement of nucleic acids in biological materials. A supplementary review.生物材料中核酸测量的最新进展。补充综述。
Analyst. 1966 Feb;91(79):78-88. doi: 10.1039/an9669100078.
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