Chapple I L C, Brock G, Eftimiadi C, Matthews J B
Unit of Periodontology, School of Dentistry, The University of Birmingham, St Chads Queensway, Birmingham B4 6NN, UK.
Mol Pathol. 2002 Dec;55(6):367-73. doi: 10.1136/mp.55.6.367.
To determine possible changes in gingival crevicular fluid (GCF) antioxidant defence in chronic adult periodontal disease and to investigate the nature of the local radical scavenging mechanisms, with particular reference to glutathione.
GCF and plasma were collected from patients with chronic periodontitis and age and sex matched control subjects (n = 10). Polymorphonuclear leucocytes (PMNLs) were prepared and gingival epithelial cells (GECs) were collected by conventional methods from periodontally healthy subjects. PMNL were stimulated with F-Met-Leu-Phe after cytochalasin B treatment. Enhanced chemiluminescence was used to determine the total antioxidant capacity and to investigate the activity of cell fractions and reducing agents. GCF concentrations of reduced (GSH) and oxidised (GSSG) glutathione were determined by high performance liquid chromatography.
Plasma and GCF from patients contained lower mean (SD) total antioxidant capacity (501.8 (123) micro M Teq/litre and 658.3 (392) micro M Teq/litre, respectively) compared with controls (577.9 (99.8) and 1351.5 (861) micro M Teq/litre, respectively). Antioxidant light recovery profiles for GCF demonstrated a stepped response, not seen in plasma, which was inhibited by N-ethylmaleimide. This response was also detected in the cytosolic fraction of GEC and anaerobically stimulated PMNL. Similar antioxidant profiles, inhibitable by N-ethylmaleimide, were obtained with cysteamine, cysteine, and GSH. Control GCF contained high mean (SD) concentrations of glutathione (GSH, 1899.8 (494.4) micro M; GSSG, 256.8 (152.4) micro M). GCF from patients with periodontitis contained significantly lower amounts of GSH (mean, 1183.1; SD, 580.3 micro M) and GSSG (mean, 150.1; SD, 44.9 micro M).
GSH values and total antioxidant capacity are reduced in chronic periodontal disease. The high concentrations of GSH present in GCF in health are similar to those found extracellularly in the lung and may represent an important antioxidant and anti-inflammatory defence strategy common to exposed epithelial surfaces.
确定慢性成人牙周病患者龈沟液（GCF）抗氧化防御的可能变化，并研究局部自由基清除机制的性质，尤其关注谷胱甘肽。
从慢性牙周炎患者以及年龄和性别匹配的对照受试者（n = 10）中收集GCF和血浆。通过常规方法从牙周健康受试者制备多形核白细胞（PMNL）并收集牙龈上皮细胞（GEC）。用细胞松弛素B处理后，用F-甲硫氨酰-亮氨酰-苯丙氨酸刺激PMNL。采用增强化学发光法测定总抗氧化能力，并研究细胞组分和还原剂的活性。通过高效液相色谱法测定还原型（GSH）和氧化型（GSSG）谷胱甘肽的GCF浓度。
与对照组（分别为577.9（99.8）和1351.5（861）μM Teq/升）相比，患者的血浆和GCF平均（SD）总抗氧化能力较低（分别为501.8（123）μM Teq/升和658.3（392）μM Teq/升）。GCF的抗氧化光恢复曲线显示出一种阶梯状反应，血浆中未见，该反应被N-乙基马来酰亚胺抑制。在GEC的胞质部分和厌氧刺激的PMNL中也检测到这种反应。用半胱胺、半胱氨酸和GSH获得了类似的抗氧化曲线，可被N-乙基马来酰亚胺抑制。对照GCF含有高平均（SD）浓度的谷胱甘肽（GSH，1899.8（494.4）μM；GSSG，256.8（152.4）μM）。牙周炎患者的GCF中GSH（平均，1183.1；SD，580.3μM）和GSSG（平均，150.1；SD，44.9μM）含量显著降低。
慢性牙周病中GSH值和总抗氧化能力降低。健康状态下GCF中高浓度的GSH与在肺细胞外发现的浓度相似，可能代表了暴露上皮表面共有的一种重要的抗氧化和抗炎防御策略。
