Aguirre N P, Vanzini V R, Torioni de Echaide S, Valentini B S, De Lucca G, Aufranc C, Canal A, Vigliocco A, Nielsen K
Instituto Nacional de Tecnologia Agropecuaria, E. E. A. Rafaela, CC 22, CP 2300, Rafaela (Santa Fe), Argentina.
J Immunoassay Immunochem. 2002;23(4):471-8. doi: 10.1081/ias-120015478.
The serological response induced by Brucella abortus strain 19 was evaluated in 52 Holstein females from a brucellosis-free herd using seven serological tests. Each calf was vaccinated at an age of 4 and 8 months old with 3 x 10(10) CFU B. abortus S19 and the antibody response was determined as the proportion of positive results to each test. The antibody dynamics, measured with the buffered plate antigen (BPA) test and the rapid automated presumptive (RAP) test, were similar. The proportion of positive reactions in these tests reached 100% one week after vaccination and remained at this level for seven weeks, after which the proportion of positive samples slowly declined to 8% (BPA) and 2% (RAP) at week 50. The response in the indirect enzyme immunoassay (i-ELISA) was similar, but shorter than that observed with the BPA/RAP. The antibody dynamic, measured using the seroagglutination test (SAT) in parallel with the 2-mercaptoethanol (2-Me) test and the complement fixation test (CFT) were similar to the RAP/BPA, but of slightly shorter duration. The competitive ELISA (c-ELISA) was positive in all animals for 3 weeks, followed by a rapid decline. The fluorescence polarization assay (FPA) reached a maximum of 68.5% positive animals at week 4 and then declined. Based on these data, the c-ELISA and FPA discriminated residual antibody activity due to vaccination more efficiently than the other tests.
使用七种血清学检测方法，对来自无布鲁氏菌病牛群的52头荷斯坦母牛接种流产布鲁氏菌19菌株后诱导的血清学反应进行了评估。每头小牛在4月龄和8月龄时分别接种3×10¹⁰CFU流产布鲁氏菌S19，抗体反应以每种检测方法的阳性结果比例来确定。用缓冲板抗原（BPA）试验和快速自动推定（RAP）试验测得的抗体动态相似。这些检测方法中的阳性反应比例在接种疫苗一周后达到100%，并在该水平维持七周，此后阳性样本比例在第50周时缓慢下降至8%（BPA）和2%（RAP）。间接酶联免疫吸附测定（i-ELISA）的反应相似，但持续时间比BPA/RAP短。与2-巯基乙醇（2-Me）试验和补体结合试验（CFT）并行使用血清凝集试验（SAT）测得的抗体动态与RAP/BPA相似，但持续时间稍短。竞争ELISA（c-ELISA）在所有动物中3周呈阳性，随后迅速下降。荧光偏振测定法（FPA）在第4周时阳性动物比例最高达到68.5%，然后下降。基于这些数据，c-ELISA和FPA比其他检测方法更有效地鉴别出因接种疫苗产生的残留抗体活性。
