Probert H M, Gibson G R
Food Microbial Sciences Unit, School of Food Biosciences, The University of Reading, Whiteknights, Reading, UK.
Lett Appl Microbiol. 2002;35(6):473-80. doi: 10.1046/j.1472-765x.2002.01223.x.
To compare the fermentation of dietary carbohydrates with reference to their prebiotic and gas-generating capacity.
Static anaerobic batch culture fermentations were carried out measuring gas generation and the prebiotic effect of five selected substrates (including various fructo-oligosaccharides, levan and maltodextrin). The largest gas producer was levan, whilst those showing no significant difference to Actilight included oligofructose and maltodextrin. Gas composition data showed that hydrogen and carbon dioxide were the two most quantitatively important gases. The substrate that appeared to have the best prebiotic effect in vitro was branched chain fructo-oligosaccharide (FOS), followed by oligofructose, Actilight and maltodextrin which each exerted a similar effect. The substrate with the least bifidogenic effect was levan.
The composition and total gas generation data showed that there was much variation between and within donor inocula. Generally, the lower gas producers had a more selective fermentation whilst larger gas producers were less specific.
The study of these three parameters enabled a more complete picture of carbohydrate breakdown to be drawn and hence highlighted the need for potential prebiotics to be more extensively evaluated in order to reduce negative side-effects such as gas distension.
比较膳食碳水化合物在益生元及产气能力方面的发酵情况。
进行静态厌氧分批培养发酵，测定5种选定底物（包括多种低聚果糖、左聚糖和麦芽糊精）的产气情况及益生元效应。产气最多的是左聚糖，而与Actilight无显著差异的包括低聚果糖和麦芽糊精。气体成分数据表明，氢气和二氧化碳是含量上最重要的两种气体。在体外似乎具有最佳益生元效应的底物是支链低聚果糖（FOS），其次是低聚果糖、Actilight和麦芽糊精，它们各自发挥相似的作用。益生元效应最小的底物是左聚糖。
成分及总产气量数据表明，供体接种物之间及内部存在很大差异。一般来说，产气较少的底物发酵更具选择性，而产气较多的底物特异性较低。
对这三个参数的研究能够更全面地描绘碳水化合物的分解情况，因此突出了需要对潜在益生元进行更广泛评估，以减少诸如腹胀等负面副作用。
