Sato Minoru, Oba Takashi, Yamaguchi Toshiyasu, Nakano Toshiki, Kahara Takashi, Funayama Katsura, Kobayashi Akio, Nakano Takahisa
Laboratory of Marine Biochemistry, Graduate School of Agricultural Science, Tohoku University, Miyagi, Japan.
Ann Nutr Metab. 2002;46(6):259-67. doi: 10.1159/000066495.
The angiotensin-I-converting enzyme (ACE) inhibitory and antihypertensive activities of wakame hydrolysates have been investigated in several studies.
Wakame (Undaria pinnatifida) was hydrolyzed using 17 kinds of proteases and the inhibitory activity of the hydrolysates for ACE was measured. Of these hydrolysates 4 with potent ACE inhibitory activity were administered singly and orally to spontaneously hypertensive rats (SHR).
The systolic blood pressure of SHR decreased significantly after single oral administration of protease S 'Amano' and proleather FG-F hydrolysates (10 mg protein/kg body weight). In a long-term feeding experiment, 7-week-old SHR were fed standard chow supplemented with protease S 'Amano'-derived wakame hydrolysates for 10 weeks. In SHR fed the 1 and 0.1% wakame hydrolysates, elevation of systolic blood pressure was still significantly suppressed for 7 weeks.
The hydrolysates derived from wakame by protease S 'Amano' have a powerful ACE-inhibitory activity (IC(50) = 86 microg protein/ml) and were effective in spite of their slight bitterness as 'physiologically functional food' with antihypertensive activity.
在多项研究中对裙带菜水解产物的血管紧张素转换酶（ACE）抑制和降压活性进行了研究。
使用17种蛋白酶对裙带菜（海带）进行水解，并测定水解产物对ACE的抑制活性。将4种具有强ACE抑制活性的水解产物单独口服给予自发性高血压大鼠（SHR）。
单次口服蛋白酶S“Amano”和皮革酶FG - F水解产物（10毫克蛋白质/千克体重）后，SHR的收缩压显著降低。在长期喂养实验中，给7周龄的SHR喂食补充了蛋白酶S“Amano”衍生的裙带菜水解产物的标准饲料10周。在喂食1%和0.1%裙带菜水解产物的SHR中，收缩压升高在7周内仍被显著抑制。
蛋白酶S“Amano”从裙带菜中获得的水解产物具有强大的ACE抑制活性（IC(50)=86微克蛋白质/毫升），尽管有轻微苦味，但作为具有降压活性的“生理功能食品”仍有效。
