Abd El Mohsen Manal M, Kuhnle Gunter, Rechner Andreas R, Schroeter Hagen, Rose Sarah, Jenner Peter, Rice-Evans Catherine A
Antioxidant Research Group, Wolfson Centre for Age-Related Diseases, GKT School of Biomedical Sciences, King's College, London, England, UK.
Free Radic Biol Med. 2002 Dec 15;33(12):1693-702. doi: 10.1016/s0891-5849(02)01137-1.
Epicatechin is a flavan-3-ol that is commonly present in green teas, red wine, cocoa products, and many fruits, such as apples. There is considerable interest in the bioavailability of epicatechin after oral ingestion. In vivo studies have shown that low levels of epicatechin are absorbed and found in the circulation as glucuronides, methylated and sulfated forms. Recent research has demonstrated protective effects of epicatechin and one of its in vivo metabolites, 3'-O-methyl epicatechin, against neuronal cell death induced by oxidative stress. Thus, we are interested in the ability of ingested epicatechin to cross the blood brain barrier and target the brain. Rats were administered 100 mg/kg body weight/d epicatechin orally for 1, 5, and 10 d. Plasma and brain extracts were analyzed by HPLC with photodiode array detection and LC-MS/MS. This study reports the presence of the epicatechin glucuronide and 3'-O-methyl epicatechin glucuronide formed after oral ingestion in the rat brain tissue.
表儿茶素是一种黄烷-3-醇，常见于绿茶、红酒、可可制品以及许多水果（如苹果）中。口服摄入后表儿茶素的生物利用度备受关注。体内研究表明，低水平的表儿茶素会被吸收，并以葡萄糖醛酸苷、甲基化和硫酸化形式存在于循环系统中。最近的研究证明了表儿茶素及其一种体内代谢物3'-O-甲基表儿茶素对氧化应激诱导的神经元细胞死亡具有保护作用。因此，我们对摄入的表儿茶素穿越血脑屏障并靶向大脑的能力感兴趣。给大鼠口服100毫克/千克体重/天的表儿茶素，持续1天、5天和10天。通过带有光电二极管阵列检测的高效液相色谱法和液相色谱-串联质谱法分析血浆和脑提取物。本研究报告了大鼠脑组织中口服摄入后形成的表儿茶素葡萄糖醛酸苷和3'-O-甲基表儿茶素葡萄糖醛酸苷的存在情况。
