通过高效液相色谱-质谱联用分析鉴定刺叶番荔枝甾体皂苷
Identification of Ruscus steroidal saponins by HPLC-MS analysis.
作者信息
de Combarieu E, Falzoni M, Fuzzati N, Gattesco F, Giori A, Lovati M, Pace R
机构信息
Indena SpA, Research and Development Laboratories, Via Don Minzoni 6, 20090 Settala (MI), Italy.
出版信息
Fitoterapia. 2002 Dec;73(7-8):583-96. doi: 10.1016/s0367-326x(02)00220-4.
A novel HPLC-UV method has been developed for the fingerprint analysis of the steroidal saponins in the rhizomes of three Ruscus species (Ruscus aculeatus, Ruscus hypoglossum and Ruscus colchicus). Saponins were identified by HPLC-ESI-MS. During the study a new major saponin was detected in the rhizomes of R. hypoglossum and R. colchicus. The structure of the new compound was defined as 1-O-[alpha-L-rhamnopyranosyl-(1-->2)-6-O-acetyl-beta-D-galactopyranosyl]-1beta,3beta,22xi,26-tetrahydroxy-furost-5(6)-en-26-O-beta-D-glucopyranoside (8) by spectral analysis.
已开发出一种新型高效液相色谱 - 紫外（HPLC - UV）方法，用于分析三种假叶树属植物（刺叶假叶树、舌叶假叶树和秋水仙假叶树）根茎中甾体皂苷的指纹图谱。通过HPLC - ESI - MS鉴定皂苷。在研究过程中，在舌叶假叶树和秋水仙假叶树的根茎中检测到一种新的主要皂苷。通过光谱分析，将新化合物的结构确定为1 - O - [α - L - 鼠李吡喃糖基 - (1→2) - 6 - O - 乙酰基 - β - D - 半乳吡喃糖基] - 1β,3β,22ξ,26 - 四羟基 - 呋甾 - 5(6) - 烯 - 26 - O - β - D - 吡喃葡萄糖苷（8）。
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