肾刷状缘膜囊泡对脯氨酸和甘氨酸的摄取
Proline and glycine uptake by renal brushborder membrane vesicles.
作者信息
McNamara P D, Ozegović B, Pepe L M, Segal S
出版信息
Proc Natl Acad Sci U S A. 1976 Dec;73(12):4521-5. doi: 10.1073/pnas.73.12.4521.
Abstract
Uptake of L-proline and glycine by rat renal brushborder membrane vesicles was seen to be osmotically sensitive, pH dependent,and occurred in the absence of proline and glycine metabolism. The uptake system for proline was Na+ gradient dependent, and exhibited a dual system for entry, Km1 = 0.067 mM and Km2 = 5.26 mM. The uptake of glycine was also Na+ gradient dependent, and exhibited a two Km system, Km1 = 0.22 mM and Km2 = 4.00 mM. Studies of proline and glycine interactions indicate a shared site which has a lower affinity and higher capacity for glycine than for proline. The high affinity glycine site and low affinity proline site do not appear to be shared.
摘要
大鼠肾刷状缘膜囊泡对L-脯氨酸和甘氨酸的摄取表现出对渗透压敏感、pH依赖性，且在脯氨酸和甘氨酸代谢不存在的情况下发生。脯氨酸的摄取系统依赖于Na+梯度，并表现出双重进入系统，Km1 = 0.067 mM，Km2 = 5.26 mM。甘氨酸的摄取也依赖于Na+梯度，并表现出双Km系统，Km1 = 0.22 mM，Km2 = 4.00 mM。脯氨酸和甘氨酸相互作用的研究表明存在一个共享位点，该位点对甘氨酸的亲和力低于脯氨酸，容量高于脯氨酸。高亲和力的甘氨酸位点和低亲和力的脯氨酸位点似乎不共享。
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本文引用的文献
1
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J Biol Chem. 1951 Nov;193(1):265-75.
2
Dibasic amino acid transport in rat-kidney cortex slices.大鼠肾皮质切片中的二价氨基酸转运
Biochim Biophys Acta. 1967 Feb 1;135(1):127-35. doi: 10.1016/0005-2736(67)90015-6.
3
Specificity of transport of neutral and basic amino acids in rat kidney.大鼠肾脏中中性和碱性氨基酸的转运特异性
Am J Physiol. 1967 Jul;213(1):185-90. doi: 10.1152/ajplegacy.1967.213.1.185.
4
The metabolic fate of [I-14C]galactitol in mammalian tissue.[I-14C]半乳糖醇在哺乳动物组织中的代谢命运。
Biochim Biophys Acta. 1968 Feb 1;156(1):9-16. doi: 10.1016/0304-4165(68)90098-6.
5
Renal tubular transport of proline, hydroxyproline, and glycine. 3. Genetic basis for more than one mode of transport in human kidney.脯氨酸、羟脯氨酸和甘氨酸的肾小管转运。3. 人类肾脏中多种转运模式的遗传基础。
J Clin Invest. 1968 Apr;47(4):823-35. doi: 10.1172/JCI105776.
6
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Am J Physiol. 1970 Jul;219(1):1-8. doi: 10.1152/ajplegacy.1970.219.1.1.
7
Separate transport systems for sugars and amino acids in developing rat kidney cortex.发育中大鼠肾皮质中糖和氨基酸的独立转运系统。
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8
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10
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Pflugers Arch. 1973 Jul 25;342(1):13-28. doi: 10.1007/BF00593247.
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