McNamara P D, Ozegović B, Pepe L M, Segal S
Proc Natl Acad Sci U S A. 1976 Dec;73(12):4521-5. doi: 10.1073/pnas.73.12.4521.
Uptake of L-proline and glycine by rat renal brushborder membrane vesicles was seen to be osmotically sensitive, pH dependent,and occurred in the absence of proline and glycine metabolism. The uptake system for proline was Na+ gradient dependent, and exhibited a dual system for entry, Km1 = 0.067 mM and Km2 = 5.26 mM. The uptake of glycine was also Na+ gradient dependent, and exhibited a two Km system, Km1 = 0.22 mM and Km2 = 4.00 mM. Studies of proline and glycine interactions indicate a shared site which has a lower affinity and higher capacity for glycine than for proline. The high affinity glycine site and low affinity proline site do not appear to be shared.
大鼠肾刷状缘膜囊泡对L-脯氨酸和甘氨酸的摄取表现出对渗透压敏感、pH依赖性，且在脯氨酸和甘氨酸代谢不存在的情况下发生。脯氨酸的摄取系统依赖于Na+梯度，并表现出双重进入系统，Km1 = 0.067 mM，Km2 = 5.26 mM。甘氨酸的摄取也依赖于Na+梯度，并表现出双Km系统，Km1 = 0.22 mM，Km2 = 4.00 mM。脯氨酸和甘氨酸相互作用的研究表明存在一个共享位点，该位点对甘氨酸的亲和力低于脯氨酸，容量高于脯氨酸。高亲和力的甘氨酸位点和低亲和力的脯氨酸位点似乎不共享。
