Paula A C B, Hayashi L S S, Freitas J C
Departamento de Farmacologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brasil.
Braz J Med Biol Res. 2003 Jan;36(1):105-12. doi: 10.1590/s0100-879x2003000100014. Epub 2002 Dec 19.
Ipomoea imperati (Convolvulaceae) lives on the sandy shores of the Brazilian coast and in other areas of the world. The anti-inflammatory activity of a methanol-water extract of the leaves of I. imperati was investigated in experimental models of acute and subchronic inflammation. Topical application of the extract (10 mg/ear) inhibited mouse ear edema induced by croton oil (89.0 +/- 1.3% by the lipid fraction with an IC50 of 3.97 mg/ear and 57.0 +/- 1.3% by the aqueous fraction with an IC50 of 3.5 mg/ear) and arachidonic acid (42.0 +/- 2.0% with an IC50 of 4.98 mg/ear and 31.0 +/- 2.0% with an IC50 of 4.72 mg/ear). Phospholipase A2, purified from Apis mellifera bee venom, was also inhibited by the extract (5.0 mg/ml lipid and aqueous fraction) in vitro in a dose-dependent manner (85% by the lipid fraction with an IC50 of 3.22 mg/ml and 25% by the aqueous fraction with an IC50 of 3.43 mg/ml). The methanol-water extract of I. imperati (1000 mg/kg) administered by the oral route also inhibited the formation of cotton pellet-induced granulomas (73.2 +/- 1.2% by the lipid fraction and 56.14 +/- 2.7% by the aqueous fraction) and did not cause gastric mucosal lesions. I. imperati extracts (10 mg/ml) also inhibited in a dose-dependent manner the muscle contractions of guinea pig ileum induced by acetylcholine and histamine (IC50 of 1.60 mg/ml for the lipid fraction and 4.12 mg/ml for the aqueous fraction). These results suggest the use of I. imperati as an anti-inflammatory and antispasmodic agent in traditional medicine.
海滨旋花（旋花科）生长在巴西海岸及世界其他地区的沙滩上。研究了海滨旋花叶甲醇 - 水提取物在急性和亚慢性炎症实验模型中的抗炎活性。局部应用该提取物（10毫克/耳）可抑制巴豆油诱导的小鼠耳肿胀（脂质部分抑制率为89.0±1.3%，IC50为3.97毫克/耳；水相部分抑制率为57.0±1.3%，IC50为3.5毫克/耳）以及花生四烯酸诱导的肿胀（抑制率分别为42.0±2.0%，IC50为4.98毫克/耳；31.0±2.0%，IC50为4.72毫克/耳）。从蜜蜂毒液中纯化的磷脂酶A2在体外也受到该提取物（脂质和水相部分均为5.0毫克/毫升）的剂量依赖性抑制（脂质部分抑制率为85%，IC50为3.22毫克/毫升；水相部分抑制率为25%，IC50为3.43毫克/毫升）。口服给予海滨旋花甲醇 - 水提取物（1000毫克/千克）也可抑制棉球诱导的肉芽肿形成（脂质部分抑制率为73.2±1.2%，水相部分抑制率为56.14±2.7%），且不会引起胃黏膜损伤。海滨旋花提取物（10毫克/毫升）还以剂量依赖性方式抑制乙酰胆碱和组胺诱导的豚鼠回肠肌肉收缩（脂质部分IC50为1.60毫克/毫升，水相部分IC50为4.12毫克/毫升）。这些结果表明海滨旋花可在传统医学中用作抗炎和解痉剂。
