Petsev Dimiter N, Chen Kai, Gliko Olga, Vekilov Peter G
Department of Chemical Engineering, University of Houston, Houston, TX 77204, USA.
Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):792-6. doi: 10.1073/pnas.0333065100. Epub 2003 Jan 24.
For critical tests of whether diffusion-limited kinetics is an option for the solution-solid phase transition of molecular substances or whether they are determined exclusively by a transition state, we performed crystallization experiments with ferritin and apoferritin, a unique pair of proteins with identical shells but different molecular masses. We find that the kinetic coefficient for crystallization is identical (accuracy <or=7%) for the pair, indicating diffusion-limited kinetics of crystallization. Data on the kinetics of this phase transition in systems ranging from small-molecule ionic to protein and viri suggest that the kinetics of solution-phase transitions for broad classes of small-molecule and protein materials are diffusion-limited.
为了严格测试扩散限制动力学是否是分子物质固 - 固相变的一种选择，或者它们是否仅由过渡态决定，我们用铁蛋白和脱铁铁蛋白进行了结晶实验，这是一对独特的蛋白质，具有相同的外壳但分子量不同。我们发现这一对蛋白质的结晶动力学系数是相同的（精度≤7%），表明结晶过程存在扩散限制动力学。从小分子离子体系到蛋白质和病毒体系的这一相变动力学数据表明，广泛种类的小分子和蛋白质材料的溶液相转变动力学是受扩散限制的。
