Herrmann Kathrin, Niedobitek Gerald
Institute for Pathology, Friedrich-Alexander University, Erlangen, Germany.
Breast Cancer Res. 2003;5(1):R13-7. doi: 10.1186/bcr561. Epub 2002 Dec 4.
Epstein-Barr virus (EBV) is a ubiquitous human gamma-herpes virus infecting more than 90% of the population worldwide. EBV is associated with certain malignancies (e.g. Burkitt lymphoma, Hodgkin lymphoma and nasopharyngeal carcinoma). Recent studies have raised the possibility that EBV may also be involved in the pathogenesis of breast carcinoma, the most common carcinoma of females. If substantiated, this finding would have major implications regarding prevention and therapy of the disease. The studies published so far have employed diverse methods, however, and the results have been controversial.
Using the EBV DNA PCR, EBV DNA in situ hybridisation and in situ hybridisation for the detection of the EBV-encoded RNAs, and using immunohistochemistry for the demonstration of the EBV-encoded nuclear antigen 1, we have studied a series of 59 invasive breast carcinomas for evidence of EBV infection.
EBV-encoded RNA-specific in situ hybridisation and EBV-encoded nuclear antigen 1 immunohistochemistry were negative in all cases. Using the PCR, EBV DNA was detected in four out of 59 cases. These cases were further studied by EBV DNA in situ hybridisation, showing an absence of viral DNA from the tumour cells.
These results indicate that breast carcinoma is not an EBV-associated tumour.
爱泼斯坦-巴尔病毒（EBV）是一种普遍存在的人类γ疱疹病毒，全球超过90%的人口受其感染。EBV与某些恶性肿瘤相关（如伯基特淋巴瘤、霍奇金淋巴瘤和鼻咽癌）。最近的研究提出了EBV可能也参与女性最常见的癌症——乳腺癌发病机制的可能性。如果这一发现得到证实，将对该疾病的预防和治疗产生重大影响。然而，迄今为止发表的研究采用了多种方法，结果存在争议。
我们使用EBV DNA聚合酶链反应（PCR）、EBV DNA原位杂交和用于检测EBV编码RNA的原位杂交，并使用免疫组织化学来显示EBV编码的核抗原1，研究了一系列59例浸润性乳腺癌，以寻找EBV感染的证据。
所有病例中EBV编码RNA特异性原位杂交和EBV编码核抗原1免疫组织化学均为阴性。使用PCR，在59例中有4例检测到EBV DNA。通过EBV DNA原位杂交对这些病例进行进一步研究，结果显示肿瘤细胞中不存在病毒DNA。
这些结果表明乳腺癌不是一种EBV相关肿瘤。
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