水溶性壳聚糖的酶法制备及其抗肿瘤活性。

Enzymic preparation of water-soluble chitosan and their antitumor activity.

作者信息

Qin Caiqin, Du Yumin, Xiao Ling, Li Zhan, Gao Xiaohai

机构信息

Department of Environmental Science, Wuhan University, 430072, Wuhan, PR China.

出版信息

Int J Biol Macromol. 2002 Dec 20;31(1-3):111-7. doi: 10.1016/s0141-8130(02)00064-8.

DOI:10.1016/s0141-8130(02)00064-8

PMID:12559434

Abstract

Water-soluble low-molecular-weight (LMW) chitosan was prepared from enzymatic hydrolysis with efficient hemicellulase. The hydrolysates were separated by ultrafiltration membranes. A separated fraction with Mw more than 5x10(3) and with a degree of deacetylation of 58% was water-soluble in the free amine form. The intraperitoneal injection of LMW chitosan and its N-acetyl product inhibited the growth of sacroma 180 (S180) tumor cells in the mice, and the maximum inhibitory rate reached 64.2%. The oral administration was also effective on decreasing weight of tumor, and the maximum inhibitory rate reached 33.7%. The Water-soluble chitosan with higher Mw than hexamer might have better antitumor activity.

摘要

采用高效半纤维素酶酶解制备了水溶性低分子量（LMW）壳聚糖。水解产物通过超滤膜进行分离。分离得到的分子量大于5×10³且脱乙酰度为58%的级分以游离胺形式可溶于水。腹腔注射LMW壳聚糖及其N - 乙酰化产物可抑制小鼠肉瘤180（S180）肿瘤细胞的生长，最大抑制率达到64.2%。口服给药对减轻肿瘤重量也有效，最大抑制率达到33.7%。分子量高于六聚体的水溶性壳聚糖可能具有更好的抗肿瘤活性。