Loughlin Michael F, Ala'Aldeen Dlawer A, Jenks Peter J
Institute of Infections and Immunity, Floor C, West Block, University Hospital, Queen's Medical Centre, University of Nottingham, UK.
J Antimicrob Chemother. 2003 Feb;51(2):367-71. doi: 10.1093/jac/dkg057.
To determine the ability of mastic monotherapy to eradicate Helicobacter pylori infection from mice.
The susceptibility of H. pylori SS1 to mastic was assessed by broth dilution determination of the MIC and MBC. Mice were inoculated intragastrically with either a suspension of H. pylori SS1 (n = 70) or brain-heart infusion broth alone (n = 10). Mice were given antimicrobial chemotherapy 4 weeks after infection and were administered the mouse equivalent of either 2 g of mastic twice daily for 7 days or a triple therapy regimen containing the mouse equivalent of 400 mg of metronidazole, 250 mg of clarithromycin and 20 mg of omeprazole twice daily for 7 days. Mice were killed either immediately or 1 month after the completion of treatment, and their stomachs cultured for H. pylori.
The mastic MIC and MBC of H. pylori SS1 were 7.80 and 31.25 mg/L, respectively. The triple therapy regimen eradicated infection from 19 of 20 SS1-infected mice. Mastic failed to eradicate infection from any of the 18 SS1-infected mice (P < 0.001) and there was no signifi- cant reduction in gastric bacterial load in mice treated with this regimen.
Despite reported beneficial effects in ulcer patients and the good in vitro activity of mastic against H. pylori, this compound is unable to eradicate H. pylori infection from mice.
确定乳香单一疗法根除小鼠幽门螺杆菌感染的能力。
通过肉汤稀释法测定最低抑菌浓度（MIC）和最低杀菌浓度（MBC），评估幽门螺杆菌SS1对乳香的敏感性。给小鼠胃内接种幽门螺杆菌SS1悬液（n = 70）或仅接种脑心浸液肉汤（n = 10）。感染4周后给予小鼠抗菌化疗，每天两次给予相当于2 g乳香的剂量，持续7天，或给予含相当于400 mg甲硝唑、250 mg克拉霉素和20 mg奥美拉唑的三联疗法方案，每天两次，持续7天。在治疗结束后立即或1个月后处死小鼠，并对其胃进行幽门螺杆菌培养。
幽门螺杆菌SS1的乳香MIC和MBC分别为7.80和31.25 mg/L。三联疗法方案根除了20只感染SS1的小鼠中的19只的感染。乳香未能根除18只感染SS1的小鼠中的任何一只的感染（P < 0.001），并且用该方案治疗的小鼠胃内细菌载量没有显著降低。
尽管有报道称乳香对溃疡患者有有益作用，且其在体外对幽门螺杆菌有良好活性，但该化合物无法根除小鼠的幽门螺杆菌感染。
