用于检测肠球菌中氨基糖苷类耐药基因的多重聚合酶链反应
Multiplex PCR for detection of aminoglycoside resistance genes in enterococci.
作者信息
Vakulenko Sergei B, Donabedian Susan M, Voskresenskiy Anatoliy M, Zervos Marcus J, Lerner Stephen A, Chow Joseph W
机构信息
Division of Infectious Diseases, Department of Biochemistry and Molecular Biology, Wayne State University, Detroit, Michigan 48201, USA.
出版信息
Antimicrob Agents Chemother. 2003 Apr;47(4):1423-6. doi: 10.1128/AAC.47.4.1423-1426.2003.
Abstract
A multiplex PCR procedure for detecting the aminoglycoside resistance genes aac(6')-Ie-aph(2")-Ia, aph(2")-Ib, aph(2")-Ic, aph(2")-Id, aph(3')-IIIa, and ant(4')-Ia was evaluated and found to determine accurately the presence of these genes in enterococci.
摘要
对一种用于检测氨基糖苷类耐药基因aac(6')-Ie-aph(2")-Ia、aph(2")-Ib、aph(2")-Ic、aph(2")-Id、aph(3')-IIIa和ant(4')-Ia的多重PCR方法进行了评估，发现该方法能准确测定肠球菌中这些基因的存在情况。
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