Hernández C J, Berríos A, Chinapen S
Department of Biology, University of Puerto Rico, Humacao Campus, CUH Station, Humacao 00791, Puerto Rico.
Comp Biochem Physiol C Toxicol Pharmacol. 2003 Apr;134(4):465-72. doi: 10.1016/s1532-0456(03)00025-5.
This study was undertaken to localize substance P-like immunoreactivity (SP) in the nerve fibers innervating the palate, identify the ganglion of the palatine nerve and determine whether it contains SP cell bodies, in the frog Rana pipiens. The palatine nerve which is a branch of the maxillo-mandibular subdivision of the trigeminal nerve was traced to the trigeminal ganglion that connects to the medulla by the trigeminal nerve root. Using an immunocytochemical method, SP containing fibers with varicosities were found in the connective tissue layer of the palate. Some of these fibers were observed adjacent to blood vessels to the epithelial layer of the palate in apparent innervation of the ciliated epithelial and mucus cells. SP-labeling was also observed in small to medium cells of the trigeminal ganglion. These results appear to support the pharmacological studies of SP on the regulation of mucociliary activity in the frog R. pipiens.
本研究旨在定位支配青蛙腭部的神经纤维中P物质样免疫反应性（SP），确定腭神经节并判断其是否含有SP细胞体。腭神经是三叉神经上颌-下颌分支的一个分支，追踪至通过三叉神经根与延髓相连的三叉神经节。采用免疫细胞化学方法，在腭部的结缔组织层发现了含SP且有膨体的纤维。其中一些纤维在靠近腭部上皮层血管处被观察到，明显支配着纤毛上皮细胞和黏液细胞。在三叉神经节的中小细胞中也观察到了SP标记。这些结果似乎支持了关于SP对青蛙牛蛙黏液纤毛活动调节的药理学研究。
