Fang Liqiong, Chai Hee-Byung, Castillo Juan J, Soejarto Djaja D, Farnsworth Norman R, Cordell Geoffrey A, Pezzuto John M, Kinghorn A Douglas
Program for Collaborative Research in the Pharmaceutical Sciences, University of Illinois at Chicago, Chicago, IL 60612, USA.
Phytother Res. 2003 May;17(5):520-3. doi: 10.1002/ptr.1315.
Phytochemical investigation of Brachistus stramoniifolius (Kunth) Miers (formerly Witheringia stramoniifolia Kunth) (Solanaceae) was initiated following primary biological screening and in view of the absence of prior phytochemical studies conducted on this species. Fractionation of an ethyl acetate extract from the roots of the plant, guided by in vitro cytotoxic activity using cultured KB (human nasopharyngeal carcinoma) cells, led to the isolation of three known compounds of the 13,14-seco-16,24-cyclosteroid type, physalins B (1), F (2) and H (3). Their structures were characterized by comparison of their physical and spectral data to published values, with 1D- and 2D-NMR experiments being performed to assure unambiguous resonance assignments. Biological evaluation of these three compounds against a panel of human and murine cancer cell lines demonstrated their broad cytotoxic activity.
鉴于此前未对曼陀罗叶短柄茄（Brachistus stramoniifolius (Kunth) Miers，原名为Witheringia stramoniifolia Kunth，茄科）进行过植物化学研究，在初步生物筛选后，我们对该植物展开了植物化学研究。以体外细胞毒性活性为导向，利用培养的KB（人鼻咽癌）细胞对该植物根部的乙酸乙酯提取物进行分离，得到了三种已知的13,14-裂-16,24-环甾体类化合物，即酸浆苦素B（1）、F（2）和H（3）。通过将其物理和光谱数据与已发表的值进行比较来确定它们的结构，并进行了一维和二维核磁共振实验以确保明确的共振归属。对这三种化合物针对一系列人和小鼠癌细胞系的生物学评价表明它们具有广泛的细胞毒性活性。
