Agin Véronique, Chichery Raymond, Maubert Eric, Chichery Marie Paule
Laboratoire de Psychophysiologie, Université de Caen, Esplanade de la paix, 14032 Caen Cedex, France.
Pharmacol Biochem Behav. 2003 Apr;75(1):141-6. doi: 10.1016/s0091-3057(03)00041-8.
When shown prawns in a glass tube, cuttlefish promptly learn to inhibit their predatory behavior and retain this ability for a long time. The cellular and molecular mechanisms of this long-term memory (LTM) are not yet known. In this study, we analyzed the dependency of LTM on de novo brain protein synthesis. Cycloheximide (CXM), a protein synthesis inhibitor, is injected intravenously immediately, 1 h, 3 h, 4 h or 6 h after the training. Retention is tested 24 h posttraining. The injections of CXM revealed one period of memory sensitivity to pharmacological intervention. CXM administered immediately or 6 h after training has no effect on LTM. Conversely, injections given between 1 and 4 h posttraining resulted in amnesia. Taken together, findings of this study establish for the first time in Sepia officinalis that de novo protein synthesis is an essential and time-dependent event for LTM formation of this form of associative learning.
当向乌贼展示玻璃管中的对虾时，它们会迅速学会抑制捕食行为，并长时间保持这种能力。这种长期记忆（LTM）的细胞和分子机制尚不清楚。在本研究中，我们分析了LTM对大脑从头合成蛋白质的依赖性。蛋白质合成抑制剂环己酰亚胺（CXM）在训练后立即、1小时、3小时、4小时或6小时静脉注射。在训练后24小时测试记忆保持情况。CXM注射显示出一个对药物干预敏感的记忆期。训练后立即或6小时注射CXM对LTM没有影响。相反，训练后1至4小时注射会导致失忆。综上所述，本研究结果首次在乌贼中证实，从头合成蛋白质是这种联想学习形式的LTM形成所必需的且具有时间依赖性的事件。
