Ndjoyi-Mbiguino Angélique, Ozouaki Francis, Legoff Jérôme, Mbopi-Kéou François-Xavier, Si-Mohamed Ali, Onas Isabelle Ndombi, Avoune Evelyne, Bélec Laurent
Laboratoire de Virologie, Département de Microbiologie, Université des Sciences de la Santé Maternité Joséphine Bongo, Gabon.
J Clin Microbiol. 2003 Jun;41(6):2662-4. doi: 10.1128/JCM.41.6.2662-2664.2003.
Asymptomatic genital shedding of herpes simplex virus type 2 (HSV-2) DNA was evidenced by real-time PCR in 25 (13.2%) of 188 cervicovaginal lavage samples and in only 13 (6.9%) paired cervicovaginal samples from 188 HSV-2-seropositive, nonpregnant childbearing-aged human immunodeficiency virus-seronegative women living in Gabon. These observations demonstrate that cervicovaginal washing is more suitable than endocervicovaginal swabbing for detecting and quantifying HSV-2 DNA by PCR in female genital secretions.
在加蓬生活的188名单纯疱疹病毒2型(HSV - 2)血清学阳性、未怀孕的育龄女性且人类免疫缺陷病毒血清学阴性的女性中,通过实时聚合酶链反应(PCR)在188份宫颈阴道灌洗样本中的25份(13.2%)以及仅13份(6.9%)配对的宫颈阴道样本中证实了HSV - 2 DNA的无症状生殖器脱落。这些观察结果表明,对于通过PCR检测和定量女性生殖器分泌物中的HSV - 2 DNA,宫颈阴道冲洗比宫颈阴道拭子采样更合适。