Blitvich Bradley J, Bowen Richard A, Marlenee Nicole L, Hall Roy A, Bunning Michel L, Beaty Barry J
Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA.
J Clin Microbiol. 2003 Jun;41(6):2676-9. doi: 10.1128/JCM.41.6.2676-2679.2003.
We evaluated the ability of epitope-blocking enzyme-linked immunosorbent assays (ELISAs) to detect West Nile virus (WNV) antibodies in domestic mammals. Sera were collected from experimentally infected horses, cats, and pigs at regular intervals and screened in ELISAs and plaque reduction neutralization tests. The diagnostic efficacies of these techniques were similar.
我们评估了表位阻断酶联免疫吸附测定(ELISA)检测家养哺乳动物西尼罗河病毒(WNV)抗体的能力。定期从实验感染的马、猫和猪身上采集血清,并在ELISA和蚀斑减少中和试验中进行筛查。这些技术的诊断效力相似。