Lima Maria E, Colpo Ana C, Salgueiro Willian G, Sardinha Guilherme E, Avila Daiana S, Folmer Vanderlei
Programa de Pós Graduação em Bioquímica, Universidade Federal do Pampa, BR 472-Km 592-Caixa Postal 118-CEP: 97500-970-Uruguaiana-RS, Brazil.
Faculdade de Farmácia, Universidade Federal do Pampa, BR 472-Km 592-Caixa Postal 118-CEP: 97500-970-Uruguaiana-RS, Brazil.
Int J Environ Res Public Health. 2014 Sep 26;11(10):10091-104. doi: 10.3390/ijerph111010091.
Recent studies have shown that phenolic compounds present in yerba mate have antioxidant defense properties. To verify whether Ilex paraguariensis extracts are capable of increasing the lifespan of an organism, we have used the free-living nematode Caenorhabditis elegans. Notably, this is the first study that analyzes the effects of the extracts of yerba mate obtained from an extraction method that mimics the manner that the plant is consumed by the population by using a live organism. Yerba mate was purchased from commercial markets from Argentina, Brazil, and Uruguay. Ilex paraguariensis extracts significantly increased the life span of C. elegans. Moreover, the extracts reduced the ROS levels per se, and protected from the reduced survival and reproduction rate induced by paraquat exposure. Considering molecular aspects, we observed that the worms pretreated with the extracts depicted higher translocation of the transcription factor DAF-16::GFP to the nucleus. However, there was no increase in the levels of the DAF-16 target genes, SOD-3 and catalase. Our results suggest that the increase of lifespan caused by the different extracts is associated to the antioxidant potential of yerba mate, however this effect is not completely mediated by daf-16.
最近的研究表明，马黛茶中存在的酚类化合物具有抗氧化防御特性。为了验证巴拉圭冬青提取物是否能够延长生物体的寿命，我们使用了自由生活的线虫秀丽隐杆线虫。值得注意的是，这是第一项通过使用活体生物，分析模拟人群食用方式的提取方法所获得的马黛茶提取物效果的研究。马黛茶购自阿根廷、巴西和乌拉圭的商业市场。巴拉圭冬青提取物显著延长了秀丽隐杆线虫的寿命。此外，提取物本身降低了活性氧水平，并保护线虫免受百草枯暴露诱导的存活率和繁殖率降低的影响。从分子层面考虑，我们观察到用提取物预处理的线虫中，转录因子DAF-16::GFP向细胞核的转位更高。然而，DAF-16靶基因SOD-3和过氧化氢酶的水平没有增加。我们的结果表明，不同提取物导致的寿命延长与马黛茶的抗氧化潜力有关，然而这种效应并不完全由daf-16介导。
