Bochoráková Hana, Paulová Hana, Slanina Jirí, Musil Pavel, Táborská Eva
Department of Biochemistry, Faculty of Medicine, Masaryk University in Brno, Brno, Czech Republic.
Phytother Res. 2003 Jun;17(6):640-4. doi: 10.1002/ptr.1216.
The content of the main flavonoids in the root of Scutellaria baicalensis Georgi cultivated in Central Europe was evaluated using the new simple RP-HPLC method with gradient of acetonitrile in mobile phase. The main components of the roots were baicalin (8.12% of dry root mass) and wogonin glucuronide (2.52%). The content of flavonoids was comparable with the content in plants cultivated in natural localities. Five main flavonoids were evaluated for their scavenging ability with DPPH radical-generating system and due to limited solubility only two flavonoids were investigated for their ability to scavenge hydroxyl radical by the aromatic hydroxylation method. The total extract was also tested in both the experimental arrangements. In experiments with DPPH, only baicalin and baicalein displayed a significant scavenging effect, while the production of OH radicals generated by UV photolysis of H(2)O(2) was considerable decreased in the presence of baicalin and wogonin glucuronide. After comparison with results obtained for the total extract, it was concluded, that the scavenging activity of the extract against DPPH is mainly derived from baicalin. On the other hand, baicalin, wogonin glucuronide and probably other flavonoids participate in scavenging OH radical.
采用流动相含乙腈梯度的新型简单反相高效液相色谱法（RP-HPLC），对在中欧种植的黄芩根中主要黄酮类化合物的含量进行了评估。根的主要成分是黄芩苷（占干根质量的8.12%）和汉黄芩苷元葡萄糖醛酸苷（2.52%）。黄酮类化合物的含量与在自然产地种植的植物中的含量相当。利用DPPH自由基产生系统评估了五种主要黄酮类化合物的清除能力，由于溶解度有限，仅通过芳香族羟基化法研究了两种黄酮类化合物清除羟基自由基的能力。在这两种实验设置中也对总提取物进行了测试。在DPPH实验中，只有黄芩苷和黄芩素表现出显著的清除效果，而在黄芩苷和汉黄芩苷元葡萄糖醛酸苷存在的情况下，过氧化氢（H₂O₂）紫外光解产生的羟基自由基的生成量显著降低。与总提取物的结果进行比较后得出结论，提取物对DPPH的清除活性主要源自黄芩苷。另一方面，黄芩苷、汉黄芩苷元葡萄糖醛酸苷以及可能的其他黄酮类化合物参与了羟基自由基的清除。
