Lejon V, Kwete J, Büscher P
Department of Parasitology, Institute of Tropical Medicine, Antwerpen, Belgium.
Trop Med Int Health. 2003 Jul;8(7):585-8. doi: 10.1046/j.1365-3156.2003.01077.x.
A pilot study was carried out on the detection of trypanosome-specific antibodies in saliva for diagnosis of sleeping sickness. All twenty-three saliva samples of parasitologically confirmed Trypanosoma brucei gambiense patients tested positive in an indirect enzyme-linked immunosorbent assay, whereas all 14 saliva samples of a negative control group remained negative. Trypanosome-specific antibody levels in patient saliva correlated with antibody levels in serum, but were about 250-fold lower. Eight of 23 undiluted saliva samples of sleeping sickness patients tested positive in CATT/T. b. gambiense and two of 23 in LATEX/T. b. gambiense. All fourteen saliva samples of the negative control group were also positive in CATT/T. b. gambiense, as were four of 14 in LATEX/T. b. gambiense. CATT and LATEX were thus inappropriate for antibody detection in saliva. These results indicate that trypanosome-specific antibody detection in saliva is possible. This could lead to the development of a simple, non-invasive, reliable saliva field test for diagnosis of sleeping sickness.
开展了一项关于检测唾液中锥虫特异性抗体以诊断昏睡病的初步研究。在间接酶联免疫吸附试验中，所有经寄生虫学确诊的布氏冈比亚锥虫病患者的23份唾液样本均呈阳性，而阴性对照组的所有14份唾液样本均为阴性。患者唾液中的锥虫特异性抗体水平与血清中的抗体水平相关，但约低250倍。23份未经稀释的昏睡病患者唾液样本中有8份在冈比亚锥虫卡片试验（CATT/T. b. gambiense）中呈阳性，23份中有2份在乳胶凝集试验（LATEX/T. b. gambiense）中呈阳性。阴性对照组的所有14份唾液样本在冈比亚锥虫卡片试验中也呈阳性，14份中有4份在乳胶凝集试验中呈阳性。因此，卡片试验和乳胶凝集试验不适用于唾液中的抗体检测。这些结果表明，唾液中锥虫特异性抗体检测是可行的。这可能会促成一种用于诊断昏睡病的简单、无创、可靠的唾液现场检测方法的开发。
