Lejon V, Jamonneau V, Solano P, Atchade P, Mumba D, Nkoy N, Bébronne N, Kibonja T, Balharbi F, Wierckx A, Boelaert M, Büscher P
Department of Parasitology, Institute of Tropical Medicine, Antwerp, Belgium.
Trop Med Int Health. 2006 May;11(5):620-7. doi: 10.1111/j.1365-3156.2006.01620.x.
The detection of trypanosome-specific antibodies in saliva is technically feasible, and, if clinically validated, could become an attractive option for non-invasive diagnosis of sleeping sickness. We wanted to optimize the test format of an enzyme-linked immunosorbent assay (ELISA)-based antibody detection system.
Different ELISA formats for antibody detection in serum and saliva were developed and standardized. Saliva and serum samples were collected from 78 patient and 128 endemic control samples, and sensitivity and specificity of saliva ELISAs, serum ELISAs and the card agglutination test for trypanosomiasis (CATT), were evaluated.
All ELISA formats showed sensitivity and specificity above 90%. Saliva ELISAs showed a similar test performance as serum ELISAs and the CATT on whole blood or serum.
This study confirms the potential of trypanosome-specific antibody detection in saliva.
检测唾液中的锥虫特异性抗体在技术上是可行的，并且如果经过临床验证，可能成为昏睡病非侵入性诊断的一个有吸引力的选择。我们希望优化基于酶联免疫吸附测定（ELISA）的抗体检测系统的测试形式。
开发并标准化了用于血清和唾液中抗体检测的不同ELISA形式。从78例患者和128例流行区对照样本中收集唾液和血清样本，并评估唾液ELISA、血清ELISA以及锥虫病卡片凝集试验（CATT）的敏感性和特异性。
所有ELISA形式的敏感性和特异性均高于90%。唾液ELISA在全血或血清上的测试性能与血清ELISA和CATT相似。
本研究证实了唾液中锥虫特异性抗体检测的潜力。
