Lin Jung Chung
Department of Microbiology, College of Medicine, Tzu Chi University, 701 Section 3, Chung Yang Road, Hualien 970, Taiwan ROC.
Antiviral Res. 2003 Jun;59(1):41-7. doi: 10.1016/s0166-3542(03)00030-5.
We report here that glycyrrhizic acid (GL), a component of licorice root (Glycyrrhiza radix), is active against EBV replication in superinfected Raji cells in a dose-dependent fashion. The IC(50) values for viral inhibition and cell growth were 0.04 and 4.8mM, respectively. The selectivity index (ratio of IC(50) for cell growth to IC(50) for viral DNA synthesis) was 120. Time of addition experiments suggested that GL interferes with an early step of EBV replication cycle (possibly penetration). GL had no effect on viral adsorption, nor did it inactivate EBV particles. Thus, GL represents a new class of anti-EBV compounds with a mode of action different from that of the nucleoside analogs that inhibit viral DNA polymerase.
我们在此报告，甘草根（Glycyrrhiza radix）的成分甘草酸（GL）对超感染的Raji细胞中的EBV复制具有剂量依赖性的活性。病毒抑制和细胞生长的IC50值分别为0.04和4.8mM。选择性指数（细胞生长的IC50与病毒DNA合成的IC50之比）为120。添加时间实验表明，GL干扰EBV复制周期的早期步骤（可能是穿透）。GL对病毒吸附没有影响，也不会使EBV颗粒失活。因此，GL代表了一类新型的抗EBV化合物，其作用方式不同于抑制病毒DNA聚合酶的核苷类似物。
