斑马鱼中的癌症遗传学与药物发现

Cancer genetics and drug discovery in the zebrafish.

作者信息

Stern Howard M, Zon Leonard I

机构信息

Howard Hughes Medical Institute, Children's Hospital of Boston, 300 Longwood Avenue, Enders 761, Boston, Massachusetts 02115, USA.

出版信息

Nat Rev Cancer. 2003 Jul;3(7):533-9. doi: 10.1038/nrc1126.

DOI:10.1038/nrc1126

PMID:12835673

Abstract

Fish have a long history of use in cancer toxicology studies, because they develop neoplasms that are histologically similar to human cancers. Because of considerable progress in zebrafish genetics and genomics over the past few years, the zebrafish system has provided many useful tools for studying basic biological processes. These tools include forward genetic screens, transgenic models, specific gene disruptions and small-molecule screens. By combining carcinogenesis assays, genetic analyses and small-molecule screening techniques, the zebrafish is emerging as a powerful system for identifying novel cancer genes and for cancer drug discovery.

摘要

鱼类在癌症毒理学研究中的应用历史悠久，因为它们会发生组织学上与人类癌症相似的肿瘤。由于过去几年斑马鱼遗传学和基因组学取得了显著进展，斑马鱼系统为研究基本生物学过程提供了许多有用的工具。这些工具包括正向遗传学筛选、转基因模型、特定基因敲除和小分子筛选。通过将致癌作用测定、遗传分析和小分子筛选技术相结合，斑马鱼正成为一种强大的系统，用于鉴定新的癌症基因和发现抗癌药物。