水烟管吸烟对斑马鱼正常发育的影响。
Effect of Water-Pipe Smoking on the Normal Development of Zebrafish.
机构信息
Department of Biomedical Sciences, College of Health Sciences, QU Health, Qatar University, Doha P.O. Box 2713, Qatar.
Biomedical Research Center, Qatar University, Doha P.O. Box 2713, Qatar.
出版信息
Int J Environ Res Public Health. 2021 Nov 6;18(21):11659. doi: 10.3390/ijerph182111659.
BACKGROUND
Among all types of tobacco consumption, Water-Pipe Smoking (WPS) is the most widely used in the Middle East and second-most in several other countries. The effect of WPS on normal development is not yet fully understood, thus the aim of this study is to explore the acute toxicity effects of WPS extract on zebrafish larvae.
METHODS
In this study, we compared the effects of WPS smoke condensates at concentrations varying from 50 to 200 µg/mL on developmental, cardiac, and behavioural (neurotoxicity) functions. Gene expression patterns of cardiac biomarkers were also evaluated by RT-qPCR.
RESULTS
Exposing zebrafish embryos to 50, 100, 150 and 200 µg/mL WPS for three days did not affect the normal morphology of Zebrafish embryos, as the tail flicking, behavioural and locomotion assays did not show any change. However, WPS deregulated cardiac markers including atrial natriuretic peptide (ANP/NPPA) and brain natriuretic peptide (BNP/NPPB). Furthermore, it induced apoptosis in a dose-dependent manner.
CONCLUSION
Our data demonstrate that WPS can significantly affect specific cardiac parameters during the normal development of zebrafish. Further investigations are necessary to elucidate the pathogenic outcome of WPS on different aspects of human life, including pregnancy.
背景
在所有类型的烟草消费中，水烟（WPS）在中东地区的使用最为广泛，在其他几个国家也位居第二。WPS 对正常发育的影响尚未完全了解，因此本研究旨在探讨 WPS 提取物对斑马鱼幼虫的急性毒性作用。
方法
在这项研究中，我们比较了浓度从 50 到 200 µg/mL 的 WPS 烟雾冷凝物对发育、心脏和行为（神经毒性）功能的影响。还通过 RT-qPCR 评估了心脏生物标志物的基因表达模式。
结果
将斑马鱼胚胎暴露于 50、100、150 和 200 µg/mL 的 WPS 中三天，不会影响斑马鱼胚胎的正常形态，因为尾巴摆动、行为和运动检测没有显示任何变化。然而，WPS 使包括心钠肽（ANP/NPPA）和脑钠肽（BNP/NPPB）在内的心脏标志物失调。此外，它还呈剂量依赖性诱导细胞凋亡。
结论
我们的数据表明，WPS 可以在斑马鱼的正常发育过程中显著影响特定的心脏参数。需要进一步研究阐明 WPS 对人类生活包括妊娠等不同方面的致病后果。
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