Atienza S G, Satovic Z, Petersen K K, Dolstra O, Martín A
Departamento de Agronomía y Mejora Genética Vegetal, Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Apdo. 4084, 14080, Córdoba, Spain.
Theor Appl Genet. 2003 Jun;107(1):123-9. doi: 10.1007/s00122-003-1220-5. Epub 2003 Mar 20.
We have developed the first quantitative trait locus (QTL) analyses for agronomic traits in a cross between F(1.1) (P1) and F(1.7) (P7) entries of Miscanthus sinensis Anderss. Both lines are offspring of the cross between MS-90-2 and MS-88-110. A map based on random amplified polymorphic DNA markers previously constructed was used to perform the QTL analyses. This map was developed using a new mapping strategy that has been designated offspring cross. Eleven QTLs were detected for height, panicle height and diameter using the programme mapqtl 4.0 and the multiple QTL method. QTL significance was determined using several analyses, including Kruskal-Wallis analyses, empirical determination of LOD critical values using permutation tests, QTLs validation with field data over 2 years and co-localization of QTLs for correlated traits. The results obtained could be the first step in developing a marker-assisted selection programming in this species for biomass production.
我们对芒草（Miscanthus sinensis Anderss.）的F(1.1)（P1）和F(1.7)（P7）品系杂交后代的农艺性状进行了首次数量性状位点（QTL）分析。这两个品系都是MS - 90 - 2和MS - 88 - 110杂交的后代。利用先前构建的基于随机扩增多态性DNA标记的图谱进行QTL分析。该图谱是采用一种新的被称为后代杂交的作图策略构建的。使用程序mapqtl 4.0和多QTL方法，检测到了与株高、穗高和直径相关的11个QTL。通过多种分析确定QTL的显著性，包括Kruskal - Wallis分析、使用置换检验经验确定LOD临界值、利用两年的田间数据验证QTL以及对相关性状的QTL进行共定位。所获得的结果可能是在该物种中开发用于生物量生产的标记辅助选择程序的第一步。
