用于产气荚膜梭菌计数的培养基的评估与改进
Evaluation and modifications of media for enumeration of Clostridium perfringens.
作者信息
Hauschild A H, Hilsheimer R
出版信息
Appl Microbiol. 1974 Jan;27(1):78-82. doi: 10.1128/am.27.1.78-82.1974.
Abstract
The suitability of the Shahidi-Ferguson perfringens, TSC (tryptose-sulfite-cycloserine), and oleandomycin-polymyxin-sulfadiazine perfringens agars for presumptive enumeration of Clostridium perfringens was tested. Of these, the TSC agar was the most satisfactory. The TSC agar method was improved by eliminating the egg yolk and using pour plates. The modified method allowed quantitative recoveries of each of 71 C. perfringens strains tested and is recommended. For confirmation of C. perfringens, the nitrite test in nitrate motility agar was unreliable, particularly after storage of the medium for a few days. In contrast, positive nitrite reactions were obtained consistently when nitrate motility agar was supplemented with glycerol and galactose.
摘要
对沙希迪-弗格森产气荚膜杆菌琼脂、TSC（胰蛋白胨-亚硫酸盐-环丝氨酸）琼脂和竹桃霉素-多粘菌素-磺胺嘧啶产气荚膜杆菌琼脂用于产气荚膜梭菌的推测计数的适用性进行了测试。其中，TSC琼脂最令人满意。通过去除蛋黄并使用倾注平板对TSC琼脂方法进行了改进。改进后的方法能对所测试的71株产气荚膜梭菌中的每一株进行定量回收，因此推荐使用。对于产气荚膜梭菌的确认，硝酸盐动力琼脂中的亚硝酸盐试验不可靠，尤其是在培养基储存几天后。相比之下，当硝酸盐动力琼脂添加甘油和半乳糖时，能持续获得阳性亚硝酸盐反应。
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本文引用的文献
1
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Appl Microbiol. 1965 Jul;13(4):559-63. doi: 10.1128/am.13.4.559-563.1965.
2
EFFECT OF PH ON TOXINOGENESIS BY CLOSTRIDIUM PERFRINGENS TYPE C.pH对C型产气荚膜梭菌产毒的影响
Can J Microbiol. 1964 Jun;10:329-44. doi: 10.1139/m64-045.
3
Quantitation of Clostridium perfringens in foods.食品中产气荚膜梭菌的定量分析。
Appl Microbiol. 1962 May;10(3):193-9. doi: 10.1128/am.10.3.193-199.1962.
4
Rapid destruction of bacteria in commonly used diluents and its elimination.常用稀释剂中细菌的快速破坏及其消除。
Appl Microbiol. 1957 Jan;5(1):21-5. doi: 10.1128/am.5.1.21-25.1957.
5
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6
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7
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Appl Microbiol. 1971 Mar;21(3):500-6. doi: 10.1128/am.21.3.500-506.1971.
8
New selective medium for the isolation of Clostridium perfringens.
Acta Microbiol Acad Sci Hung. 1969;16(3):273-8.
9
Studies on the suitability of sulphite cycloserine agar for the enumeration of clostridium perfringens in food and water.亚硫酸盐环丝氨酸琼脂用于食品和水中产气荚膜梭菌计数适用性的研究。
Zentralbl Bakteriol Orig A. 1973 May;223(4):559-61.
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