国际食品微生物标准委员会方法研究。VIII. 食品中产气荚膜梭菌计数的比较研究。

ICMSF methods studies. VIII. Comparative study for the enumeration of Clostridium perfringens in foods.

作者信息

Hauschild A H, Gilbert R J, Harmon S M, O'Keeffe M F, Vahlefeld R

出版信息

Can J Microbiol. 1977 Jul;23(7):884-92. doi: 10.1139/m77-130.

PMID:195698

Abstract

Four methods were compared in an international comparative study for the enumeration of Clostridium perfringens: the SFP (Shahidi-Ferguson perfringens) agar (A), TSC (tryptose-sulfite-cycloserin) agar (B), SC (sulfite-cycloserine) agar (C), and neomycin blood agar (D) methods. The confirmed C. perfringens counts were slightly lower for D than for A-C. The percentages of presumptive colonies confirmed as C. perfringens were essentially the same in each method. The relative numbers of nonspecific colonies were the lowest in C, followed by B, D, and A. The methods were also compared for simplicity and for aspects associated with the recognition and selection of presumptive colonies.

摘要

在一项国际比较研究中，对用于产气荚膜梭菌计数的四种方法进行了比较：沙希迪 - 弗格森产气荚膜梭菌（SFP）琼脂法（A）、胰蛋白胨 - 亚硫酸盐 - 环丝氨酸（TSC）琼脂法（B）、亚硫酸盐 - 环丝氨酸（SC）琼脂法（C）和新霉素血琼脂法（D）。经确认的产气荚膜梭菌计数，方法D略低于方法A - C。每种方法中，被确认为产气荚膜梭菌的推测菌落百分比基本相同。非特异性菌落的相对数量在方法C中最低，其次是方法B、D和A。还对这些方法在简易程度以及与推测菌落的识别和选择相关的方面进行了比较。