Jiang Hongmei, Doerge R W, Gelvin Stanton B
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392, USA.
Plant J. 2003 Jul;35(2):219-36. doi: 10.1046/j.1365-313x.2003.01796.x.
Agrobacterium tumefaciens is a plant pathogen that incites crown gall tumors by transferring to and expressing a portion of a resident plasmid in plant cells. Currently, little is known about the host response to Agrobacterium infection. Using suppressive subtractive hybridization and DNA macroarrays, we identified numerous plant genes that are differentially expressed during early stages of Agrobacterium-mediated transformation. Expression profiling indicates that Agrobacterium infection induces plant genes necessary for the transformation process while simultaneously repressing host defense response genes, thus indicating successful utilization of existing host cellular machinery for genetic transformation purposes. A comparison of plant responses to different strains of Agrobacterium indicates that transfer of both T-DNA and Vir proteins modulates the expression of host genes during the transformation process.
根癌土壤杆菌是一种植物病原体，它通过将自身质粒的一部分转移到植物细胞并在其中表达，从而引发冠瘿瘤。目前，对于宿主对根癌土壤杆菌感染的反应了解甚少。利用抑制性消减杂交和DNA宏阵列技术，我们鉴定出了许多在根癌土壤杆菌介导的转化早期阶段差异表达的植物基因。表达谱分析表明，根癌土壤杆菌感染诱导了转化过程所需的植物基因，同时抑制了宿主防御反应基因，这表明根癌土壤杆菌成功利用了宿主现有的细胞机制来实现遗传转化。对植物对不同根癌土壤杆菌菌株反应的比较表明，T-DNA和Vir蛋白的转移在转化过程中均会调节宿主基因的表达。
