Dailianis S, Domouhtsidou G P, Raftopoulou E, Kaloyianni M, Dimitriadis V K
Department of Zoology, Aristotle University of Thessaloniki, Thessaloniki, 54006, Greece.
Mar Environ Res. 2003 Oct;56(4):443-70. doi: 10.1016/S0141-1136(03)00005-9.
The neutral red lysosomal retention assay (NRR) of the haemocytes, and the acetylcholinesterase activity (AChE) in the haemolymph, the digestive gland, the gills and the mantle/gonad complex have been evaluated on mussels Mytilus galloprovincialis collected from Thermaikos and Strymonikos gulfs (northern Greece) in June and October 2001. The validity of performing the above core biomarkers is supported, firstly by their ability to respond to different pollution levels and, secondly, by the significant linear correlation among them. The evaluation of the micronuclei frequency (MN) has been performed in gill tissue and haemocytes of the same mussels and, according to the results, it needs more research in order its use as stress indices to be validated. In addition, the first results on cAMP levels in the gills, the mantle/gonad complex and the digestive gland, whose concentrations correlated to both, NRR and AChE introduce this signal transduction molecule as a new, promising biomarker.
对2001年6月和10月从希腊北部的塞尔迈湾和斯特里蒙湾采集的加利福尼亚贻贝血细胞进行了中性红溶酶体保留试验（NRR），并评估了其血淋巴、消化腺、鳃以及外套膜/性腺复合体中的乙酰胆碱酯酶活性（AChE）。首先，上述核心生物标志物对不同污染水平的响应能力，其次，它们之间显著的线性相关性，支持了进行上述生物标志物检测的有效性。对同一批贻贝的鳃组织和血细胞进行了微核频率（MN）评估，根据结果，将其用作应激指标还需要更多研究来验证。此外，在鳃、外套膜/性腺复合体和消化腺中cAMP水平的初步结果表明，其浓度与NRR和AChE均相关，这将这种信号转导分子作为一种新的、有前景的生物标志物引入。
