Rautio Merja, Eerola Erkki, Väisänen-Tunkelrott Marja-Liisa, Molitoris Denise, Lawson Paul, Collins Matthew D, Jousimies-Somer Hannele
National Public Health Institute (KTL), Department of Microbiology, Anaerobe Reference Laboratory, Helsinki, Finland.
Syst Appl Microbiol. 2003 Jun;26(2):182-8. doi: 10.1078/072320203322346029.
During studies on the bacteriology of appendicitis in children, we often isolated from inflamed and non-inflamed tissue samples, an unusual bile-resistant pigment-producing strictly anaerobic gram-negative rod. Phenotypically this organism resembles members of Bacteroides fragilis group of species, as it is resistant to bile and exhibits a special-potency-disk pattern (resistance to vancomycin, kanamycin and colistin) typical for the B. fragilis group. However, the production of brown pigment on media containing haemolysed blood and a cellular fatty acid composition dominated by iso-C15:0, suggests that the organism most closely resembles species of the genus Porphyromonas. However, the unidentified organism differs from porphyromonads by being bile-resistant and by not producing butyrate as a metabolic end-product. Comparative 16S ribosomal RNA gene sequencing studies show the unidentified organism represents a distinct sub-line, associated with but distinct from, the miss-classified species Bacteroides putredinis. The clustering of the unidentified bacterium with Bacteroides putredinis was statistically significant, but they displayed > 4% sequence divergence with each other. Chromosomal DNA-DNA pairing studies further confirmed the separateness of the unidentified bacterium and Bacteroides putredinis. Based on phenotypic and phylogenetic considerations, it is proposed that Bacteroides putredinis and the unidentified bacterium from human sources be classified in a new genus Alistipes, as Alistipes putredinis comb. nov. and Alistipes finegoldii sp. nov., respectively. The type strain of Alistipes finegoldii is CCUG 46020(T) (= AHN243(T)).
在对儿童阑尾炎细菌学的研究过程中，我们经常从发炎和未发炎的组织样本中分离出一种不寻常的、产生胆汁抗性色素的严格厌氧革兰氏阴性杆菌。从表型上看，这种微生物类似于脆弱拟杆菌属的成员，因为它对胆汁具有抗性，并且呈现出脆弱拟杆菌属特有的特殊药敏纸片模式（对万古霉素、卡那霉素和黏菌素耐药）。然而，在含有溶血血液的培养基上产生棕色色素以及细胞脂肪酸组成以异-C15:0为主，这表明该微生物与卟啉单胞菌属的物种最为相似。然而，这种未鉴定的微生物与卟啉单胞菌不同，它具有胆汁抗性，并且不产生丁酸作为代谢终产物。比较16S核糖体RNA基因测序研究表明，这种未鉴定的微生物代表一个独特的亚系，与误分类的腐败拟杆菌相关但又不同。未鉴定细菌与腐败拟杆菌的聚类在统计学上具有显著性，但它们彼此之间的序列差异>4%。染色体DNA-DNA配对研究进一步证实了未鉴定细菌与腐败拟杆菌的分离。基于表型和系统发育方面的考虑，建议将来自人类来源的腐败拟杆菌和未鉴定细菌分别归入一个新属——艾利斯菌属，即分别为新组合的腐败艾利斯菌和新种费氏艾利斯菌。费氏艾利斯菌的模式菌株是CCUG 46020(T)（=AHN243(T)）。
