de Roodt Adolfo Rafael, Litwin Silvana, Vidal Juan Carlos
Instituto Nacional de Producción de Biológicos-ANLIS, Dr Carlos G Malbrán, Av Vélez Sarsfield 563, CP 1281, Buenos Aires, Argentina.
Toxicon. 2003 Jun;41(8):949-58. doi: 10.1016/s0041-0101(02)00392-6.
The changes in hemorrhagic activity, proteolytic activity on gelatin and the lethal potency of four Bothrops venoms treated at different pH values or with EDTA were studied. Venoms from B. alternatus, B. jararaca, B. moojeni and B. neuwiedii of Argentina were preincubated at pH 5.8, 5.1 or 3.8 or with EDTA and the hemorrhagic activity expressed as size of the hemorrhagic lesion or as the amount of hemoglobin extracted, the proteolytic activity on gelatin and the lethal potency were determined. Although the MHDs recorded in rats were 19-56 fold higher than those recorded in mice, the A(550) extracted per gram of hemorrhagic haloes was very similar in rats or mice independent of the venom dose. Inhibition of proteolytic activity after preincubation at pH 5.1 or 3.8, agrees with the decreased amount of hemoglobin extracted from the hemorrhagic haloes, and with the increase in mean survival time after the i.p. injection to mice. Preincubation with EDTA resulted in 80% inhibition of hemorrhagic activity of B. jararaca venom and complete inhibition with the other Bothrops venoms tested. Measurement of the amount of hemoglobin extracted gives significant information in comparative studies, not available by measurement of the size of hemorrhagic haloes.
研究了四种矛头蝮属蛇毒在不同pH值下或用乙二胺四乙酸（EDTA）处理后出血活性、对明胶的蛋白水解活性和致死效力的变化。来自阿根廷的交替矛头蝮、巴西矛头蝮、穆氏矛头蝮和纳氏矛头蝮的蛇毒在pH 5.8、5.1或3.8下预孵育，或用EDTA处理，然后测定以出血性病变大小或提取的血红蛋白量表示的出血活性、对明胶的蛋白水解活性和致死效力。尽管在大鼠中记录的最小致死剂量（MHDs）比在小鼠中记录的高19 - 56倍，但每克出血晕圈中提取的A(550)在大鼠或小鼠中非常相似，与蛇毒剂量无关。在pH 5.1或3.8下预孵育后蛋白水解活性的抑制，与从出血晕圈中提取的血红蛋白量减少以及腹腔注射给小鼠后的平均存活时间增加一致。用EDTA预孵育导致巴西矛头蝮蛇毒的出血活性抑制80%，而在所测试的其他矛头蝮属蛇毒中则完全抑制。提取的血红蛋白量的测定在比较研究中提供了重要信息，这是通过测量出血晕圈大小无法获得的。
