六价铬诱导的DNA氧化损伤：电镀工人尿中8-羟基脱氧鸟苷浓度（8-OHdG）升高。
Chromium (VI) induced oxidative damage to DNA: increase of urinary 8-hydroxydeoxyguanosine concentrations (8-OHdG) among electroplating workers.
作者信息
Kuo H W, Chang S F, Wu K Y, Wu F Y
机构信息
Institute of Environmental Health, China Medical College, Taichung,Taiwan, ROC.
出版信息
Occup Environ Med. 2003 Aug;60(8):590-4. doi: 10.1136/oem.60.8.590.
AIMS
To investigate the concentration of urinary 8-hydroxydeoxyguanosine (8-OHdG) among electroplating workers in Taiwan.
METHODS
Fifty workers were selected from five chromium (Cr) electroplating plants in central Taiwan. The 20 control subjects were office workers with no previous exposure to Cr. Urinary 8-OHdG concentrations were determined using high performance liquid chromatography with electrochemical detection.
RESULTS
Urinary 8-OHdG concentrations among Cr workers (1149.5 pmol/kg/day) were higher than those in the control group (730.2 pmol/kg/day). There was a positive correlation between urinary 8-OHdG concentrations and urinary Cr concentration (r = 0.447, p < 0.01), and urinary 8-OHdG correlated positively with airborne Cr concentration (r = 0.285). Using multiple regression analysis, the factors that affected urinary 8-OHdG concentrations were alcohol, the common cold, and high urinary Cr concentration. There was a high correlation of urinary 8-OHdG with both smoking and drinking, but multiple regression analysis showed that smoking was not a significant factor. Age and gender were also non-significant factors.
CONCLUSION
8-OHdG, which is an indicator of oxidative DNA damage, was a sensitive biomarker for Cr exposure.
目的
调查台湾电镀工人尿中8-羟基脱氧鸟苷（8-OHdG）的浓度。
方法
从台湾中部的五家镀铬电镀厂选取50名工人。20名对照对象为以前未接触过铬的办公室工作人员。采用高效液相色谱电化学检测法测定尿中8-OHdG浓度。
结果
铬作业工人尿中8-OHdG浓度（1149.5 pmol/kg/天）高于对照组（730.2 pmol/kg/天）。尿中8-OHdG浓度与尿铬浓度呈正相关（r = 0.447，p < 0.01），且尿中8-OHdG与空气中铬浓度呈正相关（r = 0.285）。采用多元回归分析，影响尿中8-OHdG浓度的因素有饮酒、感冒和尿铬浓度高。尿中8-OHdG与吸烟和饮酒均高度相关，但多元回归分析表明吸烟不是一个显著因素。年龄和性别也不是显著因素。
结论
8-OHdG作为氧化DNA损伤的指标，是铬暴露的敏感生物标志物。
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