Li Taisheng, Qiu Zhifeng, Han Yang, Wang Zhong, Fan Hongwei, Lu Wei, Xie Jing, Ma Xiaojun, Wang Aixia
Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
Chin Med J (Engl). 2003 Jul;116(7):985-7.
To study the alteration of peripheral lymphocyte subsets in severe acute respiratory syndrome (SARS) patients and to help improve the early diagnosis of the disease.
Anti-coagulating blood samples from 98 SARS patients in the acute phase, 56 normal healthy blood donors, and from patients infected by HIV, CMV and EBV were collected. The T lymphocyte subsets were counted by flow cytometry using fluorescence-labeled specific monoclonal antibodies.
A significant decrease was observed in all SARS patients in their peripheral CD4(+) and CD8(+) T lymphocyte absolute counts [256 (104) x 10(6)/L and 256 (117) x 10(6)/L, respectively], which were also lower than those of the patients infected with HIV, CMV and EBV. All patients infected with HIV, CMV and EBV had significantly higher CD8(+) T lymphocyte counts in comparison with normal controls.
Decrease of both CD4(+) and CD8(+) T lymphocytes of patients is related to onset of SARS. T lymphocyte subset analysis would help improve the early diagnosis of the disease.
研究严重急性呼吸综合征（SARS）患者外周血淋巴细胞亚群的变化，以协助改善该疾病的早期诊断。
采集98例急性期SARS患者、56例正常健康献血者以及感染HIV、CMV和EBV患者的抗凝血液样本。使用荧光标记的特异性单克隆抗体通过流式细胞术计数T淋巴细胞亚群。
所有SARS患者外周血CD4(+)和CD8(+) T淋巴细胞绝对计数均显著降低[分别为256(104)×10(6)/L和256(117)×10(6)/L]，且低于感染HIV、CMV和EBV的患者。与正常对照组相比，所有感染HIV、CMV和EBV的患者CD8(+) T淋巴细胞计数均显著更高。
SARS患者CD4(+)和CD8(+) T淋巴细胞均减少与SARS发病有关。T淋巴细胞亚群分析有助于改善该疾病的早期诊断。
