Denner Ewald B M, Smith Garriet W, Busse Hans-Jürgen, Schumann Peter, Narzt Thomas, Polson Shawn W, Lubitz Werner, Richardson Laurie L
Institut für Mikrobiologie und Genetik, Universität Wien, A-1030 Vienna, Austria.
Department of Biology and Geology, University of South Carolina Aiken, Aiken, SC, USA.
Int J Syst Evol Microbiol. 2003 Jul;53(Pt 4):1115-1122. doi: 10.1099/ijs.0.02359-0.
A bacterium previously isolated from a diseased colony of the scleractinian coral Dichocoenia stokesi (common name elliptical star coral) was subjected to a detailed polyphasic taxonomic characterization. The isolate, designated WP1T, was halophilic and strictly aerobic and formed golden-orange-pigmented colonies after prolonged incubation. Cells of WP1T were gram-negative, rod-shaped and showed a characteristic branching rod morphology. Chemotaxonomically, WP1T was characterized by having Q-10 as the major respiratory lipoquinone and sym-homospermidine as the main component of the cellular polyamine content. The predominant constituent in the cellular fatty acid profile was C18:1 omega7c, along with C19:0 cyclo omega8c and C16:0. Other fatty acids present in smaller amounts were C17:0, C18:0, C16:1 omega7c, C20:1 omega7c and C18:1 2-OH. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. Minor amounts of diphosphatidylglycerol, phosphatidylmonomethylethanolamine and phosphatidyldimethylethanolamine were present. The G + C content of the genomic DNA was 66.3 mol%. Phylogenetic analysis of the 16S rRNA gene sequence showed that WP1T represents a separate subline of descent within the order 'Rhizobiales' of the 'Alphaproteobacteria'. The new line of descent falls within the group of families that includes the Rhizobiaceae, Bartonellaceae, Brucellaceae and 'Phyllobacteriaceae', with no particular relative within this group. The 16S rRNA gene sequence similarity to all established taxa within this group was not higher than 92.0% (to Mesorhizobium mediterraneum). To accommodate this emerging coral pathogen, the creation of a new genus and species is proposed, Aurantimonas coralicida gen. nov., sp. nov. (type strain WP1T = CIP 107386T = DSM 14790T).
一株先前从患病的石珊瑚目椭圆星珊瑚（学名：Dichocoenia stokesi）群体中分离出的细菌，经过了详细的多相分类学特征鉴定。该分离株命名为WP1T，嗜盐且严格需氧，长时间培养后形成金橙色色素的菌落。WP1T细胞革兰氏阴性，杆状，呈现出特征性的分支杆状形态。化学分类学上，WP1T的特征是具有Q-10作为主要呼吸脂醌，以及共生高亚精胺作为细胞多胺含量的主要成分。细胞脂肪酸谱中的主要成分是C18:1 ω7c，以及C19:0环ω8c和C16:0。其他含量较少的脂肪酸有C17:0、C18:0、C16:1 ω7c、C20:1 ω7c和C18:1 2-OH。主要极性脂为磷脂酰乙醇胺、磷脂酰甘油和磷脂酰胆碱。存在少量二磷脂酰甘油、磷脂酰单甲基乙醇胺和磷脂酰二甲基乙醇胺。基因组DNA的G + C含量为66.3 mol%。16S rRNA基因序列的系统发育分析表明，WP1T代表了“变形菌门”“根瘤菌目”内一个独立的进化分支。这个新的进化分支属于包括根瘤菌科、巴尔通体科、布鲁氏菌科和“叶杆菌科”的科群，在该科群内没有特别近缘的种。与该科群内所有已确定分类单元的16S rRNA基因序列相似性不高于92.0%（与地中海中生根瘤菌相比）。为容纳这种新出现的珊瑚病原体，建议创建一个新属和新种，即珊瑚橙单胞菌（Aurantimonas coralicida），新属，新种（模式菌株WP1T = CIP 107386T = DSM 14790T）。
