Vourli S, Tzouvelekis L S, Tzelepi E, Lebessi E, Legakis N J, Miriagou V
Laboratory of Bacteriology, Hellenic Pasteur Institute, Vas. Sofias 127, Athens 11521, Greece.
FEMS Microbiol Lett. 2003 Aug 8;225(1):149-53. doi: 10.1016/S0378-1097(03)00510-X.
A class 1 integron, In111, carried by a self-transferable plasmid from an Escherichia coli clinical strain was characterized. The variable region of In111 constituted an array of gene cassettes encoding the extended-spectrum beta-lactamase IBC-1, the aminoglycoside-modifying enzymes AAC(6')-Ib and ANT(3")-Ia, dihydrofolate reductase I and a putative polypeptide (SMR-2) sharing similarity with the Qac transporters. Transcription of the gene cassettes was driven by a hybrid-type P1 promoter located in a typical 5' conserved segment (CS). The 3'CS included sulI, qacEDelta1, orf5 and orf6. In111 was bounded on the right by an inversely oriented IRt. The 5'CS was preceded by an intact IS26 element followed by an aphA1 gene.
对来自大肠杆菌临床菌株的一个可自我转移质粒携带的1类整合子In111进行了特性分析。In111的可变区由一系列基因盒组成，这些基因盒编码超广谱β-内酰胺酶IBC-1、氨基糖苷修饰酶AAC(6')-Ib和ANT(3")-Ia、二氢叶酸还原酶I以及一种与Qac转运蛋白具有相似性的推定多肽(SMR-2)。基因盒的转录由位于典型5'保守区段(CS)的杂合型P1启动子驱动。3'CS包括sulI、qacEΔ1、orf5和orf6。In111右侧由反向排列的IRt界定。5'CS之前是一个完整的IS26元件，后面跟着一个aphA1基因。
