Oliveira Laerte, Paiva Paulo B, Paiva Antonio C M, Vriend Gerrit
Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.
Proteins. 2003 Sep 1;52(4):553-60. doi: 10.1002/prot.10489.
Sequence entropy-variability plots based on alignments of very large numbers of sequences-can indicate the location in proteins of the main active site and modulator sites. In the previous article in this issue, we applied this observation to a series of well-studied proteins and concluded that it was possible to detect most of the residues with a known functional role. Here, we apply the method to rhodopsin-like G protein-coupled receptors. Our conclusion is that G protein binding is the main evolutionary constraint on these receptors, and that other ligands, such as agonists, act as modulators. The activation of the receptors can be described as a simple, two-step process, and the residues involved in signal transduction can be identified.
基于大量序列比对的序列熵变图——可以指示蛋白质中主要活性位点和调节剂位点的位置。在本期的上一篇文章中，我们将这一观察结果应用于一系列经过充分研究的蛋白质，并得出结论，即有可能检测到大多数具有已知功能作用的残基。在这里，我们将该方法应用于视紫红质样G蛋白偶联受体。我们的结论是，G蛋白结合是这些受体的主要进化限制因素，而其他配体，如激动剂，则作为调节剂起作用。受体的激活可以描述为一个简单的两步过程，并且可以识别参与信号转导的残基。
