Dufner Danielle, Previs Stephen F
Department of Nutrition, D-201, Case Western Reserve University School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106-4906, USA.
Curr Opin Clin Nutr Metab Care. 2003 Sep;6(5):511-7. doi: 10.1097/00075197-200309000-00004.
Our goal is to explain how 2H(2)O, a stable isotope of water, can be used to quantify metabolic flux in vivo. Although 2H(2)O was originally used more than 65 years ago, recent reports have described new applications. Investigators have also refined the original work. We discuss those points, emphasizing factors that should be considered when interpreting data. As our goal is to discuss why 2H(2)O is a novel tracer for studying metabolism, we do not comment on the interpretation(s) of physiological data.
It is clear that 2H(2)O can now be used to address questions related to carbohydrate, lipid, protein and DNA synthesis. A particular advantage of using 2H(2)O is that investigators can study metabolism in free-living individuals. Using this novel tracer method, it is thus possible to elucidate new, highly relevant, knowledge regarding health and disease.
As the use of 2H(2)O requires few assumptions, the application of this tracer should yield sound information regarding the regulation of biochemical reactions in vivo.
我们的目标是解释水的稳定同位素2H₂O如何用于体内代谢通量的定量分析。尽管2H₂O在65多年前就已首次使用，但近期报告描述了其新的应用。研究人员也对最初的工作进行了改进。我们讨论这些要点，重点强调在解释数据时应考虑的因素。由于我们的目标是讨论为什么2H₂O是研究代谢的新型示踪剂，因此我们不对生理数据的解释进行评论。
显然，2H₂O现在可用于解决与碳水化合物、脂质、蛋白质和DNA合成相关的问题。使用2H₂O的一个特别优势在于研究人员能够在自由活动的个体中研究代谢。利用这种新型示踪方法，因而有可能阐明有关健康和疾病的新的、高度相关的知识。
由于使用2H₂O所需的假设较少，这种示踪剂的应用应该能够提供有关体内生化反应调节的可靠信息。
