Franke Dirk, Machajewski Timothy, Hsu Che-Chang, Wong Chi-Huey
Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
J Org Chem. 2003 Aug 22;68(17):6828-31. doi: 10.1021/jo030021m.
Two methods have been developed for the highly efficient enzymatic synthesis of L-fructose: one is based on rhamnulose-1-phosphate aldolase and acid phosphatase using racemic glyceraldehyde and dihydroxyacetone phosphate as substrates; the other is to generate enantiomerically pure L-glyceraldehyde in situ from glycerol for the aldol reaction, using galactose oxidase catalyzed oxidation of glycerol in the presence of catalase. Using this four-enzyme system, enantiomerically pure L-fructose was obtained. Using the more expensive dihydroxyacetone phosphate, the yield was 55% after purification.
已经开发出两种用于高效酶促合成L-果糖的方法：一种是基于鼠李糖-1-磷酸醛缩酶和酸性磷酸酶，使用外消旋甘油醛和磷酸二羟丙酮作为底物；另一种是在过氧化氢酶存在下，利用半乳糖氧化酶催化甘油氧化，从甘油原位生成对映体纯的L-甘油醛用于醛醇反应。使用这个四酶系统，获得了对映体纯的L-果糖。使用更昂贵的磷酸二羟丙酮，纯化后的产率为55%。
