利用嗜热栖热菌 HB8 的 L-岩藻糖-1-磷酸醛缩酶（FucA）合成稀有糖。
Synthesis of rare sugars with L-fuculose-1-phosphate aldolase (FucA) from Thermus thermophilus HB8.
机构信息
National Glycoengineering Research Center and the State Key Laboratory of Microbial Technology, Shandong University, Jinan, Shandong 250100, China.
出版信息
Bioorg Med Chem Lett. 2011 Sep 1;21(17):5084-7. doi: 10.1016/j.bmcl.2011.03.072. Epub 2011 Mar 23.
Abstract
We report herein a one-pot four-enzyme approach for the synthesis of the rare sugars d-psicose, d-sorbose, l-tagatose, and l-fructose with aldolase FucA from a thermophilic source (Thermus thermophilus HB8). Importantly, the cheap starting material DL-GP (DL-glycerol 3-phosphate), was used to significantly reduce the synthetic cost.
摘要
我们在此报告了一种一锅四酶法，用于合成罕见的糖 d-阿洛酮糖、d-山梨糖、l-塔格糖和 l-果糖，所用酶来自嗜热源（Thermus thermophilus HB8）的醛缩酶 FucA。重要的是，廉价的起始原料 DL-GP（DL-甘油 3-磷酸）被用于显著降低合成成本。
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