Henry Carolyn J, Tyler Jeff W, McEntee Margaret C, Stokol Tracy, Rogers Kenita S, Chun Ruthanne, Garrett Laura D, McCaw Dudley L, Higginbotham Mary Lynn, Flessland Karen A, Stokes Pam Koffend
Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA.
Am J Vet Res. 2003 Aug;64(8):1017-20. doi: 10.2460/ajvr.2003.64.1017.
To evaluate the veterinary version of the bladder tumor antigen (V-BTA) test as a screening test for transitional cell carcinoma (TCC) of the lower urinary tract of dogs.
229 client-owned dogs.
Urine samples from dogs were shipped overnight to a single laboratory to facilitate testing within 48 hours of collection by use of the V-BTA rapid latex agglutination urine dipstick test. Groups of dogs included the following: 1) dogs with TCC of the lower urinary tract, 2) healthy control dogs, 3) unhealthy control dogs with non-TCC urinary tract disease, and 4) unhealthy control dogs without urinary tract disease. Test sensitivity and specificity were calculated by use of standard methods. Logistic models were developed to assess the effect of disease status, test conditions, urine composition, and signalment on the performance of the V-BTA test.
A total of 229 urine samples were analyzed, including 48 from dogs with suspected (n = 3) or confirmed (45) TCC. Test sensitivities were 88, 87, and 85% for all dogs with (suspected and confirmed) TCC, dogs with confirmed TCC at any site, and dogs with confirmed TCC of the urinary bladder, respectively. Test specificities were 84, 41, and 86% for healthy control dogs, unhealthy control dogs with non-TCC urinary tract disease, and unhealthy control dogs without urinary tract disease, respectively. The test performed slightly better on centrifuged urine samples than on uncentrifuged urine samples.
Our results indicate that the V-BTA test is useful in screening for urinary tract TCC in dogs.
评估兽用膀胱肿瘤抗原（V - BTA）检测作为犬下泌尿道移行细胞癌（TCC）筛查试验的效果。
229只客户拥有的犬。
将犬的尿液样本隔夜运送至单个实验室，以便在采集后48小时内使用V - BTA快速乳胶凝集尿试纸条检测进行检测。犬的分组如下：1）患有下泌尿道TCC的犬；2）健康对照犬；3）患有非TCC泌尿道疾病的非健康对照犬；4）无泌尿道疾病的非健康对照犬。采用标准方法计算检测的敏感性和特异性。建立逻辑模型以评估疾病状态、检测条件、尿液成分和特征对V - BTA检测性能的影响。
共分析了229份尿液样本，其中48份来自疑似（n = 3）或确诊（45）TCC的犬。所有（疑似和确诊）患有TCC的犬、任何部位确诊患有TCC的犬以及膀胱确诊患有TCC的犬的检测敏感性分别为88%、87%和85%。健康对照犬、患有非TCC泌尿道疾病的非健康对照犬和无泌尿道疾病的非健康对照犬的检测特异性分别为84%、41%和86%。该检测在离心尿液样本上的表现略优于未离心的尿液样本。
我们的结果表明，V - BTA检测可用于犬泌尿道TCC的筛查。
