Vandecasteele Stefaan Johan, Peetermans Willy Eduard, Merckx Rita, Van Eldere Johan
Infectious Diseases Research Group, Department of Microbiology and Immunology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium.
J Infect Dis. 2003 Sep 1;188(5):730-7. doi: 10.1086/377452. Epub 2003 Aug 4.
The expression of the genes icaA, icaC, aap, and atlE--with a putative role in the pathogenesis of Staphylococcus epidermidis foreign body infections--and of mecA and 3 housekeeping genes (gmk, tpi, and hsp60) was examined in vitro and in vivo. In vitro expression levels of ica, atlE, and gmk were higher in sessile than in planktonic bacteria. Exposure to foreign bodies in vitro and in vivo induced a sharp increase in ica expression that was followed by a progressive decrease. The in vivo expression of aap and mecA was high during early but low during late foreign body infections. The in vivo expression of atlE and gmk remained relatively high and stable. In conclusion, biofilm genes encoding for structural elements are mainly expressed during early foreign body infections. The ica genes are associated with initial colonization but not with persistence. The constant expression of atlE and gmk may indicate a role during the entire course of foreign body infections.
研究了icaA、icaC、aap和atlE基因（推测在表皮葡萄球菌异物感染发病机制中起作用）以及mecA和3个管家基因（gmk、tpi和hsp60）在体外和体内的表达情况。ica、atlE和gmk在体外生物被膜菌中的表达水平高于浮游菌。体外和体内接触异物均诱导ica表达急剧增加，随后逐渐下降。aap和mecA在异物感染早期的体内表达较高，但在后期较低。atlE和gmk的体内表达保持相对较高且稳定。总之，编码结构元件的生物膜基因主要在异物感染早期表达。ica基因与初始定植有关，但与持续存在无关。atlE和gmk的持续表达可能表明它们在异物感染的整个过程中发挥作用。
