Mariano Priscilla de Laet Sant'Ana, Milan Eveline Pípolo, da Matta Daniel Archimedes, Colombo Arnaldo Lopes
Laboratório Especial de Micologia, Universidade Federal de São Paulo, São Paulo, SP, 04023-062, Brasil.
Mem Inst Oswaldo Cruz. 2003 Jun;98(4):533-8. doi: 10.1590/s0074-02762003000400020. Epub 2003 Aug 18.
We investigated the presence of Candida dubliniensis among isolates previously identified as Candida albicans and maintained in a yeast stock collection from 1994 to 2000. All isolates were serotyped and further evaluated for antifungal susceptibility profile. After doing a screening test for C. dubliniensis isolates based on the capability of colonies to grow at 42 C, its final identification was obtained by randomly amplified polymorphic DNA (RAPD) analysis using three different primers. A total of 46 out of 548 screened isolates did not exhibit growth at 42 C and were further genotyped by RAPD. Eleven isolates were identified as C. dubliniensis with RAPD analysis. Regarding serotypes, 81.5% of C. albicans and all C. dubliniensis isolates belonged to serotype A. Of note, 9 out of 11 C. dubliniensis isolates were obtained from patients with acquired immunodeficiency syndrome (Aids) and all of them were susceptible to azoles and amphotericin B. We found 17 (3%) C. albicans isolates that were dose-dependent susceptibility or resistant to azoles. In conclusion, we found a low rate of C. dubliniensis isolates among stock cultures of yeasts previously identified as C. albicans. Most of these isolates were recovered from oral samples of Aids patients and exhibited high susceptibility to amphotericin B and azoles. C. albicans serotype A susceptible to all antifungal drugs is the major phenotype found in our stock culture.
我们调查了1994年至2000年保存在酵母菌种库中先前鉴定为白色念珠菌的分离株中都柏林念珠菌的存在情况。对所有分离株进行血清分型，并进一步评估其抗真菌药敏谱。在基于菌落能否在42℃生长对都柏林念珠菌分离株进行筛选试验后，通过使用三种不同引物的随机扩增多态性DNA（RAPD）分析进行最终鉴定。在548株筛选的分离株中，共有46株在42℃不生长，并通过RAPD进一步进行基因分型。通过RAPD分析鉴定出11株为都柏林念珠菌。关于血清型，81.5%的白色念珠菌和所有都柏林念珠菌分离株属于A血清型。值得注意的是，11株都柏林念珠菌分离株中有9株来自获得性免疫缺陷综合征（艾滋病）患者，并且它们对唑类和两性霉素B均敏感。我们发现17株（3%）白色念珠菌分离株对唑类呈剂量依赖性敏感或耐药。总之，我们在先前鉴定为白色念珠菌的酵母库存培养物中发现都柏林念珠菌分离株的比例较低。这些分离株大多从艾滋病患者的口腔样本中分离得到，并且对两性霉素B和唑类表现出高度敏感性。对所有抗真菌药物敏感的A血清型白色念珠菌是我们库存培养物中发现的主要表型。
