Denda M, Hori J, Koyama J, Yoshida S, Nanba R, Takahashi M, Horii I, Yamamoto A
Shiseido Research Center, Yokohama, Japan.
Arch Dermatol Res. 1992;284(6):363-7. doi: 10.1007/BF00372041.
Stratum corneum sphingolipids are of particular importance in maintaining the water permeability barrier of mammalian epidermis. Free amino acids also play an important role in water retention in the stratum corneum. To clarify the way in which these substances affect scaly skin, stratum corneum sphingolipids and free amino acids collected from artificially-induced scaly skin were analysed. Scaly skin was induced by tape stripping. The total amount of sphingolipids was quantified by gas chromatography and five of sphingolipid fractions were isolated and quantified by thin-layer chromatography. Free amino acids were analysed using a high-speed amino analyser. The total amount of sphingolipid in scaly skin did not differ statistically from that in control skin. However, a significant change in the distribution of the five sphingolipid species was observed in scaly skin and the total amount of amino acids was decreased in scaly skin. These results suggest that the distribution of these five types of sphingolipid and the total amount of amino acids are responsible for scaly skin.
角质层鞘脂在维持哺乳动物表皮的水渗透屏障方面尤为重要。游离氨基酸在角质层的保水方面也起着重要作用。为了阐明这些物质影响皮肤干燥起鳞屑的方式，对从人工诱导的皮肤干燥起鳞屑样本中收集的角质层鞘脂和游离氨基酸进行了分析。皮肤干燥起鳞屑是通过胶带剥离诱导产生的。鞘脂的总量通过气相色谱法定量，五个鞘脂组分通过薄层色谱法分离并定量。游离氨基酸使用高速氨基酸分析仪进行分析。皮肤干燥起鳞屑样本中的鞘脂总量与对照皮肤相比无统计学差异。然而，在皮肤干燥起鳞屑样本中观察到五种鞘脂种类的分布有显著变化，并且皮肤干燥起鳞屑样本中的氨基酸总量减少。这些结果表明，这五种鞘脂的分布和氨基酸总量与皮肤干燥起鳞屑有关。
