Ohasi K, Ueda H, Yamazaki M, Kimura S, Abe S, Yamaguchi H
Research Institute, Nichinichi Food Co., Mie, Japan.
Yakugaku Zasshi. 1992 Dec;112(12):919-25. doi: 10.1248/yakushi1947.112.12_919.
The biological activity of a preparation of heat killed cells of Enterococcus faecalis, FK-23 which was isolated from the feces of a healthy human, was investigated in C3H/He mice. Intraperitoneal injection of the preparation caused an accumulation of neutrophils and macrophages in the peritoneal cavity of the mice 6 h later. As a parameter of the activation of macrophages, the effect of the FK-23 preparation on the production of tumor necrosis factor (TNF) was examined. The mice were given two consecutive intravenous injections of the preparation at a dose of 10 micrograms/mouse and, 3 h later, of 300 micrograms/mouse. The TNF level in the sera reached 99 U/ml in mice 2 h after the second injection. This preparation also stimulated peritoneal macrophages to produce TNF in vitro and increased the capacity of neutrophils to adhere to plastic plates and to release active oxygens, but did not induce blastogenic transformation of lymphocytes. These results suggest that the FK-23 preparation is a biological response modifier (BRM) with various activities on phagocytes similar to a streptococcal antitumor agent, OK432.
对从健康人体粪便中分离出的粪肠球菌热灭活细胞制剂FK - 23的生物活性，在C3H/He小鼠中进行了研究。腹腔注射该制剂6小时后，小鼠腹腔内出现中性粒细胞和巨噬细胞聚集。作为巨噬细胞活化的一个参数，检测了FK - 23制剂对肿瘤坏死因子（TNF）产生的影响。给小鼠连续两次静脉注射该制剂，剂量分别为10微克/只和300微克/只，间隔3小时。第二次注射后2小时，小鼠血清中的TNF水平达到99 U/ml。该制剂还能在体外刺激腹腔巨噬细胞产生TNF，并增强中性粒细胞黏附于塑料平板及释放活性氧的能力，但不诱导淋巴细胞的增殖转化。这些结果表明，FK - 23制剂是一种生物反应调节剂（BRM），对吞噬细胞具有多种活性，类似于链球菌抗肿瘤制剂OK432。
